20 copies of the UAS
site are fused upstream of the shi1
coding region (this encodes a temperature-sensitive dominant negative form of shi
). A small intron derived from the Mhc
gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene has been inserted between the promoter and coding sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the coding sequence.