Allele Dmel\Ack^Scer\UAS.cSb

General Information
Symbol	Dmel\Ack^Scer\UAS.cSb	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct b of Schoenherr	FlyBase ID	FBal0270230
Feature type	allele	Associated gene	Dmel\Ack

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Schoenherr et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of wild-type Ack. (Schoenherr et al., 2012)
Carried in construct	P{UAS-Ack.Sb} (Schoenherr et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^GMR.PU (Schoenherr et al., 2012)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PU (Schoenherr et al., 2012) interommatidial bristle \| supernumerary, with Scer\GAL4^GMR.PU (Schoenherr et al., 2012)
Detailed Description
	Statement Reference Flies expressing one copy of Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] have slightly larger eyes than normal, while flies expressing two copies of Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] have a highly disorganised eye. Expression of Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] results in an increase in the number of interommatidial bristles without significantly affecting the number of pigment cells when assessed at 42% pupa development. (Schoenherr et al., 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU is a suppressor of lethal \| pharate adult stage phenotype of Scer\GAL4^GMR.PU, W^GMR.PG, yki^KK109756 (Schoenherr et al., 2012) Ack^Scer\UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of increased cell death phenotype of W^GMR.PG/W^GMR.PG (Schoenherr et al., 2012) Ack^Scer\UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of rpr^GMR.PH (Schoenherr et al., 2012) Ack^Scer\UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of W^Ala5.GMR (Schoenherr et al., 2012) Ack^Scer\UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of W^GMR.PG/W^GMR.PG (Schoenherr et al., 2012) aos[+], aos^Δ7, Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of W^Ala5.GMR (Schoenherr et al., 2012) RasGAP1^mip-w+, Ack^Scer\UAS.cSb, Gap1[+], Scer\GAL4^GMR.PU is a suppressor of visible phenotype of W^Ala5.GMR (Schoenherr et al., 2012)
Other
Statement Reference Ack^Scer\UAS.cSb, Hsap\YAP1^{S94A.S127A.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype (Schoenherr et al., 2012) Ack^Scer\UAS.cSb, Hsp83^GD1202, Scer\GAL4^GMR.PU, W^Ala5.GMR has lethal phenotype (Schoenherr et al., 2012) Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU, W^Ala5.GMR, yki^KK109756 has lethal phenotype (Schoenherr et al., 2012)
Phenotype Manifest In
Suppressor of
Statement Reference Ack^Scer\UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of eye disc phenotype of W^GMR.PG/W^GMR.PG (Schoenherr et al., 2012) Ack^Scer\UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of rpr^GMR.PH (Schoenherr et al., 2012) Ack^Scer\UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of W^Ala5.GMR (Schoenherr et al., 2012) Ack^Scer\UAS.cSb/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of W^GMR.PG/W^GMR.PG (Schoenherr et al., 2012) aos[+], aos^Δ7, Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of W^Ala5.GMR (Schoenherr et al., 2012) RasGAP1^mip-w+, Ack^Scer\UAS.cSb, Gap1[+], Scer\GAL4^GMR.PU is a suppressor of eye phenotype of W^Ala5.GMR (Schoenherr et al., 2012)
Other
Statement Reference Ack^Scer\UAS.cSb, Hsap\YAP1^{S94A.S127A.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype (Schoenherr et al., 2012) Ack^Scer\UAS.cSb, RasGAP1^mip-w+/Gap1[+], Scer\GAL4^GMR.PU has pigment cell \| supernumerary phenotype (Schoenherr et al., 2012) Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU, aos[+]/aos^Δ7 has pigment cell \| supernumerary phenotype (Schoenherr et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Expression of Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] greatly suppresses the reduced eye size caused by W[GMR.PG]. Expression of Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] suppresses the reduced eye size caused by rpr[GMR.PH]. Co-expression of Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] suppresses the increased number of apoptotic cells seen posterior to the second mitotic wave and in the posterior region of the eye disc in the zone of compensatory proliferation in eye discs expressing W[GMR.PG]. Expression of Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] suppresses the small eye phenotype caused by W[Ala5.GMR]. Further suppression is seen if the flies are also heterozygous for either aos[Δ7]/+ or Gap1[mip-w+]/+. Animals expressing Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] in a aos[Δ7]/+ or Gap1[mip-w+]/+ background show a 25% increase in pigment cell number when assessed at 42% pupa development. Expression of Ack[Scer\UAS.cSb] under the control of Scer\GAL4[GMR.PU] suppresses the small eye phenotype caused by W[Ala5.GMR]. Further suppression is seen if the flies are also co-expressing SH3PX1[KK100618]. Co-expression of either Hsp26[KK106347] or Magi[GD10066] further reduces the size of the eye in a W[Ala5.GMR], Ack[Scer\UAS.cSb], Scer\GAL4[GMR.PU] background. Co-expression of drk[KK109233] in a W[Ala5.GMR], Ack[Scer\UAS.cSb], Scer\GAL4[GMR.PU] background results in flies with eyes that are greatly reduced in size. Co-expression of Ack[Scer\UAS.cSb] and yki[Scer\UAS.cHa] under the control of Scer\GAL4[GMR.PU] results in a massively overgrown eye.Co-expression of Ack[Scer\UAS.cSb] and yki[Scer\UAS.cHa] under the control of Scer\GAL4[GMR.PU] results in a massively overgrown eye. (Schoenherr et al., 2012)
Xenogenetic Interactions
Statement Reference Co-expression of Ack[Scer\UAS.cSb] and Hsap\YAP1[S94A.S127A.Scer\UAS] under the control of Scer\GAL4[GMR.PU] results in an increased eye size. (Schoenherr et al., 2012)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Ack^Scer\UAS.cSb
Name Synonym	Saccharomyces cerevisiae UAS construct b of Schoenherr
Secondary FlyBase IDs

References ( 1 )
Research paper	Schoenherr et al., 2012, PLoS Genet. 8(5): e1002725 Drosophila activated cdc42 kinase has an anti-apoptotic function. [FBrf0218372]

Allele Dmel\AckScer\UAS.cSb

Allele Dmel\Ack^Scer\UAS.cSb