Allele Dmel\velo^LL05209

General Information
Symbol	Dmel\velo^LL05209	Species	D. melanogaster
Name		FlyBase ID	FBal0270271
Feature type	allele	Associated gene	Dmel\velo

Allele class
Mutagen	piggyBac transposase
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	piggyBac transposase (Berdnik et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference PBac{SAstopDsRed}LL05207 is inserted in the fifth intron of the long transcript of velo. (Berdnik et al., 2012)
Caused by insertion	PBac{SAstopDsRed}LL05209 (Berdnik et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (Berdnik et al., 2012) neuroanatomy defective \| somatic clone (Berdnik et al., 2012)
Phenotype Manifest In
	adult antennal lobe projection neuron DL1 adPN \| somatic clone (Berdnik et al., 2012) antennal lobe glomerulus DL1 \| somatic clone (Berdnik et al., 2012) dendrite \| somatic clone (Berdnik et al., 2012)
Detailed Description
	Statement Reference velo[LL05209] mutants exhibit projection neuron dendrite targeting defects. velo[LL05209] mutant neuroblast clones exhibit several defects. First, the number of neurons is reduced from an average of 35 ad projection neurons in wild-type to 5 neurons in velo[LL05209] mutant clones. Second, the overall dendritic mass is reduced and disorganised. Third, dendrites aberrantly innervate the wrong glomeruli within the antennal lobe, or ectopically project outside the antennal lobe. velo[LL05209] mutant dorsolateral glomerulus DL1 neurons exhibit various dendrite mistargeting defects in the antennal lobe. In adults, these phenotypes can be grouped into five distinct phenotypic classes. In class 1, dendrites innervated DL1 sparsely by spill over into incorrect adjacent areas. In class 2, dendrites are found in the dorsolateral region of the antennal lobe, but excluded from the DL1 glomerulus. Class 3 mutant DL1 projection neurons project to ventromedial regions in the antennal lobe, preferably but not exclusively to the VM6 or VC3 glomeruli. Class 4 mutant DL1 projection neurons mistarget their dendrites outside the antennal lobe mostly into the subesophageal ganglion. Class 5 mutant dendrites innervate multiple glomeruli within the antennal lobe. In 96% of velo[LL05209] mutant DL1 axons 2 or fewer collaterals innervate the mushroom body calyx. In 78% of velo[LL05209] mutant axons the dorsal branch is missing. velo[LL05209] mutant DL1 projection neurons exhibit defects in axon morphology. Axons extend as wild-type and always reach the end of the lateral horn, but only form 0-2 collaterals in the mushroom body calyx, and often have a missing or shorter dorsal branch in the lateral horn. These axonal phenotypes are independent of the phenotypic class of dendrite mistargeting. Approximately 38% of velo[LL05209] DL1 projection neurons exhibit ventromedially mistargeted dendrites. (Berdnik et al., 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference velo^LL05209 has neuroanatomy defective \| somatic clone phenotype, suppressible by lwr¹³/lwr[+] (Berdnik et al., 2012) velo^LL05209 has neuroanatomy defective \| somatic clone phenotype, suppressible by smt3^ex77/smt3[+] (Berdnik et al., 2012)
Phenotype Manifest In
Suppressed by
Statement Reference velo^LL05209 has adult antennal lobe projection neuron DL1 adPN \| somatic clone phenotype, suppressible by lwr¹³/lwr[+] (Berdnik et al., 2012) velo^LL05209 has adult antennal lobe projection neuron DL1 adPN \| somatic clone phenotype, suppressible by smt3^ex77/smt3[+] (Berdnik et al., 2012) velo^LL05209 has antennal lobe glomerulus DL1 \| somatic clone phenotype, suppressible by lwr¹³/lwr[+] (Berdnik et al., 2012) velo^LL05209 has antennal lobe glomerulus DL1 \| somatic clone phenotype, suppressible by smt3^ex77/smt3[+] (Berdnik et al., 2012) velo^LL05209 has dendrite \| somatic clone phenotype, suppressible by lwr¹³/lwr[+] (Berdnik et al., 2012) velo^LL05209 has dendrite \| somatic clone phenotype, suppressible by smt3^ex77/smt3[+] (Berdnik et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference A smt3[ex77] or lwr[13] heterozygous background suppress the dendritic mistargeting seen in velo[LL05209] mutant DL1 projection neurons. (Berdnik et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	velo^LL05209 is rescued by Scer\GAL4^GH146/velo^{Scer\UAS.T:Ivir\HA1} (Berdnik et al., 2012)
Comments	MARCM overexpression of velo[Scer\UAS.T:Ivir\HA1] rescues velo[LL05209] mutant dendrite and axon phenotypes. Approximately 91% of velo[LL05209] mutant DL1 projection neurons correctly innervate the DL1 glomerulus with concomitant expression of one copy of velo[Scer\UAS.T:Ivir\HA1]. In 96% of velo[LL05209] mutant DL1 axons 2 or fewer collaterals innervate the mushroom body calyx. Upon introduction of velo[Scer\UAS.T:Ivir\HA1], only 3% of examined DL1 axons have 2 or fewer collaterals in the mushroom body calyx whereas 97% have 3 or more collaterals. (Berdnik et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Based on dendrite targeting defects in the antennal lobe, the following velo alleles can be ranked from most severe to weakest as follows: velo[e01260] > velo[LL05209] > velo[LL05207]. (Berdnik et al., 2012)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	velo^LL05209
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Berdnik et al., 2012, J. Neurosci. 32(24): 8331--8340 The SUMO Protease Verloren Regulates Dendrite and Axon Targeting in Olfactory Projection Neurons. [FBrf0218602]

Allele Dmel\veloLL05209

Allele Dmel\velo^LL05209