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General Information
Symbol
Dmel\rigNIG.11171R
Species
D. melanogaster
Name
FlyBase ID
FBal0270766
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a 500bp inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of rigNIG.11171R under the control of Scer\GAL4Mef2.PR has no effect on flight ability of adult flies, mobility of the third instar larvae or shape of pupae and Scer\GAL4elav-C155-driven expression does not impair adult viability.

Expression of rigNIG.11171R driven by Scer\GAL4Mef2.PR (without UAS-dicer2 to enhance RNAi efficiency) does not result in flight or lifespan defects.

Flies co-expressing two different insertions of rigNIG.11171R (P{NIG.11171R}1 and P{NIG.11171R}2) ubiquitously under the control of Scer\GAL41032.hx, Scer\GAL4da.PU or Scer\GAL4Act5C.PU are completely lethal. When expressed in muscle lineages under the control of Scer\GAL4Mef2.PR viable flies are seen at both 25[o]C and 29[o]C. When Dcr-2Scer\UAS.cDa is also co-expressed almost complete lethality is seen at 29[o]C. Flies co-expressing Dcr-2Scer\UAS.cDa at 25[o]C show a progressive decline in flight ability. Complete lethality is seen at both 25[o]C and 29[o]C when rigNIG.11171R is expressed under the control of two further muscle drivers, Scer\GAL4c179 or Scer\GAL4how-24B. Expression of rigNIG.11171R in the somatic musculature under the control of Scer\GAL4G7 results in viability at 25[o]C but semi-lethality at 29[o]C. Pan-neuronal expression under the control of Scer\GAL4elav.PU results in viability at 25[o]C, both in the presence or absence of Dcr-2Scer\UAS.cDa. Flies are viable at 29[o]C in the absence of Dcr-2Scer\UAS.cDa, but complete lethality is seen when it is present. Flies co-expressing Dcr-2Scer\UAS.cDa at 25[o]C show flight and climbing defects compared to controls. Some flies have curved, rather than straight wing blades and in some cases the wings are crumpled and unopened. Expression in motor neurons under the control of Scer\GAL4RapGAP1-OK6 is viable at 25[o]C, in the absence of Dcr-2Scer\UAS.cDa, and semi-viable flies when it is present. Flies are semi-lethal at 29[o]C in the absence of Dcr-2Scer\UAS.cDa, but completely lethal when it is present.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Simultaneous expression of Gem3ΔN.Scer\UAS and rigNIG.11171R under the control of Scer\GAL4Mef2.PR leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium.

Co-expression of rigNIG.11171R and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in lethality.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of rigScer\UAS.T:Avic\GFP-ECFP rescues the lethality seen when rigNIG.11171R is expressed under the control of Scer\GAL41032.hx.

Expression of rigΔN.Scer\UAS fails to rescue the lethality seen when rigNIG.11171R is expressed under the control of Scer\GAL41032.hx.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
rigNIG.11171R
Name Synonyms
Secondary FlyBase IDs
    References (4)