FlyBase Allele Report: Dmel\Smox[NIG.2262R]

General Information

Symbol

Dmel\Smox^NIG.2262R

Species

D. melanogaster

Name

FlyBase ID

FBal0273106

Feature type

allele

Associated gene

Dmel\Smox

Associated Insertion(s)

Carried in Construct

P{NIG.2262R}

Also Known As

UAS-Smad2-i

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.2262R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-2262R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^AB1

(Hevia et al., 2017)

abnormal size | adult stage, with Scer\GAL4^Dll-md23

(Hevia et al., 2017)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

decreased cell number | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

increased cell size | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

Phenotype Manifest In

leg, with Scer\GAL4^Dll-md23

(Hevia et al., 2017)

nucleus | adult stage, with Scer\GAL4^AB1

(Hevia et al., 2017)

salivary gland | adult stage, with Scer\GAL4^AB1

(Hevia et al., 2017)

wing, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

wing vein | ectopic, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

Detailed Description

Statement

Reference

The expression of Smox^NIG.2262R under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) leads to the adult wing showing a significant decrease in size, associated with a decrease in cell number and with a significant increase in cell size, as compared to controls; these wing also develop ectopic veins around the longitudinal L5 vein. Expression of Smox^NIG.2262R also leads to significant decreases of the adult leg size (Scer\GAL4^Dll-md23-driven expression + Dicer-2) and of the salivary gland nucleus size (Scer\GAL4^AB1-driven expression + Dicer-2), as compared to controls.

(Hevia et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, Smox^NIG.2262R has abnormal size | adult stage phenotype, enhanceable by CycE^JF02473, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has abnormal size | adult stage phenotype, enhanceable by Scer\GAL4^nub.PU/stg^NIG.1395R

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has abnormal size | adult stage phenotype, enhanceable by yki^GD11187/Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has abnormal size | adult stage phenotype, enhanceable by InR^K1409A.UAS, Scer\GAL4^nub.PU

(Hevia et al., 2017)

NOT Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, Smox^NIG.2262R has decreased cell number | adult stage phenotype, non-enhanceable by InR^K1409A.UAS, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, Smox^NIG.2262R has abnormal size | adult stage phenotype, suppressible by hpo^KK101704/Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has abnormal size | adult stage phenotype, suppressible by Pten^GD13500, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has increased cell size | adult stage phenotype, suppressible by InR^K1409A.UAS, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has increased cell size | adult stage phenotype, suppressible by Pten^GD13500, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has decreased cell number | adult stage phenotype, suppressible by Pten^GD13500, Scer\GAL4^nub.PU

(Hevia et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^nub.PU, Smox^NIG.2262R has abnormal size | adult stage phenotype, non-suppressible by CycE^UAS.cUa, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has abnormal size | adult stage phenotype, non-suppressible by stg^UAS.cNa/Scer\GAL4^nub.PU

(Hevia et al., 2017)

Enhancer of

Statement

Reference

Smox^NIG.2262R, Scer\GAL4^nub.PU is an enhancer of abnormal size | adult stage phenotype of InR^K1409A.UAS, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Smox^NIG.2262R/Scer\GAL4^nub.PU is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, yki^GD11187

(Hevia et al., 2017)

Suppressor of

Statement

Reference

Smox^NIG.2262R/Scer\GAL4^nub.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, hpo^KK101704

(Hevia et al., 2017)

Smox^NIG.2262R, Scer\GAL4^nub.PU is a suppressor | partially of abnormal size | adult stage phenotype of Pten^GD13500, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Smox^NIG.2262R, Scer\GAL4^nub.PU is a suppressor of decreased cell size | adult stage phenotype of Pten^GD13500, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Smox^NIG.2262R, Scer\GAL4^nub.PU is a suppressor of increased cell number | adult stage phenotype of Pten^GD13500, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, enhanceable by CycE^JF02473, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, enhanceable by InR^K1409A.UAS, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, enhanceable by Scer\GAL4^nub.PU/stg^NIG.1395R

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, enhanceable by yki^GD11187/Scer\GAL4^nub.PU

(Hevia et al., 2017)

NOT Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, non-enhanceable by InR^K1409A.UAS, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, suppressible by Pten^GD13500, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, suppressible by InR^K1409A.UAS, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, suppressible by hpo^KK101704/Scer\GAL4^nub.PU

(Hevia et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, non-suppressible by CycE^UAS.cUa, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Scer\GAL4^nub.PU, Smox^NIG.2262R has wing phenotype, non-suppressible by stg^UAS.cNa/Scer\GAL4^nub.PU

(Hevia et al., 2017)

Enhancer of

Statement

Reference

Smox^NIG.2262R, Scer\GAL4^nub.PU is an enhancer of wing phenotype of InR^K1409A.UAS, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Smox^NIG.2262R/Scer\GAL4^nub.PU is an enhancer of wing phenotype of Scer\GAL4^nub.PU, yki^GD11187

(Hevia et al., 2017)

Suppressor of

Statement

Reference

Smox^NIG.2262R/Scer\GAL4^nub.PU is a suppressor of wing phenotype of Scer\GAL4^nub.PU, hpo^KK101704

(Hevia et al., 2017)

Smox^NIG.2262R, Scer\GAL4^nub.PU is a suppressor | partially of wing phenotype of Pten^GD13500, Scer\GAL4^nub.PU

(Hevia et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased wing size induced by the expression of Smox^NIG.2262R under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) is enhanced by the co-expression of either CycE^JF02473 or stg^NIG.1395R, but is not suppressed by the co-expression of either CycE^Scer\UAS.cUa or stg^Scer\UAS.cNa.

The double co-expression either of Smox^NIG.2262R and yki^GD11187 or of Smox^NIG.2262R and InR^{K1409A.Scer\UAS}, under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi), leads to a more severe decrease in wing size compared to all corresponding single expression conditions. The co-expression of InR^{K1409A.Scer\UAS} suppresses the increased wing cell size, but does not affect the decreased wing cell number, induced by the expression of Smox^NIG.2262R under the control of Scer\GAL4^nub.PU.

The double co-expression either of hpo^KK101704 and Smox^NIG.2262R or of Pten^GD13500 and Smox^NIG.2262R under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) suppresses the wing defects induced by the single expression conditions.

(Hevia et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

2262R-1

P{NIG.2262R}1

2262R-2

P{NIG.2262R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Smox^NIG.2262R

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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