FlyBase Allele Report: Dmel\Adck1[NIG.3608R]

General Information

Symbol

Dmel\Adck1^NIG.3608R

Species

D. melanogaster

Name

FlyBase ID

FBal0273884

Feature type

allele

Associated gene

Dmel\Adck1

Associated Insertion(s)

Carried in Construct

P{NIG.3608R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.3608R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-3608R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^PO-36.UAS

(Park et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mhc.PU

(Yoon et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

abnormal size | third instar larval stage, with Scer\GAL4^Sgs3.PD

(Yoon et al., 2019)

decreased body size | adult stage, with Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

flightless, with Scer\GAL4^Mhc.PU

(Yoon et al., 2019)

flightless, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

increased cell death | adult stage, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

lethal - all die before end of P-stage, with Scer\GAL4^Mef2.PU

(Yoon et al., 2019)

lethal - all die before end of P-stage, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

short lived, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

viable, with Scer\GAL4^Cg.PU

(Yoon et al., 2019)

viable, with Scer\GAL4^Mhc.PU

(Yoon et al., 2019)

viable, with Scer\GAL4^nSyb.PU

(Yoon et al., 2019)

visible, with Scer\GAL4^Mhc.PU

(Yoon et al., 2019)

visible, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

Phenotype Manifest In

adult somatic muscle cell of thorax, with Scer\GAL4^Mhc.PU

(Yoon et al., 2019)

adult somatic muscle cell of thorax, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

mitochondrion | adult stage, with Scer\GAL4^Mhc.PU

(Yoon et al., 2019)

mitochondrion | adult stage, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

mitochondrion | third instar larval stage, with Scer\GAL4^Sgs3.PD

(Yoon et al., 2019)

salivary gland | third instar larval stage, with Scer\GAL4^Sgs3.PD

(Yoon et al., 2019)

wing, with Scer\GAL4^Mhc.PU

(Yoon et al., 2019)

wing, with Scer\GAL4^Tub.PU

(Yoon et al., 2019)

Detailed Description

Statement

Reference

Flies expressing Adck1^NIG.3608R under the control of either Scer\GAL4^Tub.PU (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) or Scer\GAL4^Mhc.PU (but not Scer\GAL4^Cg.PU or Scer\GAL4^nSyb.PU) show defective "held-up" wing position phenotypes, reduced locomotor activity and slower walking speed than controls.

In flies expressing Adck1^NIG.3608R under the control of either Scer\GAL4^Tub.PU (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) or Scer\GAL4^Mhc.PU, thoracic muscles are abnormally oriented and show deformed mitochondrial morphologies. Additionally, expression under the control of Scer\GAL4^Tub.PU induces cell death in thoracic muscles.

Expressing Adck1^NIG.3608R under the control of Scer\GAL4^Sgs3.PD results in mitochondria with a larger area than controls in salivary glands of third instar larvae.

(Yoon et al., 2019)

Scer\GAL4^Ilp2.PR-mediated expression results in a significant reduction in the body weight of emerging adults compared to controls.

(Hasygar and Hietakangas, 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Adck1^NIG.3608R, Scer\GAL4^Tub.PU has short lived phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^Tub.PU