FB2025_04 , released October 2, 2025
Allele: Dmel\CCT4NIG.5525R
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General Information
Symbol
Dmel\CCT4NIG.5525R
Species
D. melanogaster
Name
FlyBase ID
FBal0274718
Feature type
allele
Associated gene
Dmel\CCT4
Associated Insertion(s)
Carried in Construct
P{NIG.5525R}
Also Known As
5525R
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
in vitro construct
(Japanese National Institute of Genetics, 2012.5.21)
Progenitor genotype
Carried in construct
P{NIG.5525R}
Cytology
Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-5525R
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing CCT4NIG.5525R under the control of Scer\GAL4ey.PU results in complete loss of eye disc field (with no effect on antennal field) in third instar larvae which, in turn, leads to the complete absence of head structures (but normal thorax and abdomen) in pharate adults.

      Expressing CCT4NIG.5525R under the control of Scer\GAL4en.PU results in reduced cell proliferation and an almost complete loss of the wing disc posterior compartment, while expression under the control of either Scer\GAL4Bx-MS1096 or Scer\GAL4nub.PU results in severely malformed wings.

      Expressing CCT4NIG.5525R under the control of Scer\GAL4ptc.PU leads to increased cell death in the wing pouch, a narrower anterior-posterior compartment boundary of the wing disc and greatly reduced salivary glands in third instar larvae compared to controls. In adults, the number and size of cells in the wing blade domain between L3 and L4 veins and the overall domain size are significantly reduced compared to wild-type controls.

      Expressing CCT4NIG.5525R under the control of Scer\GAL4GMR.PU fails to induce cell death in the posterior compartment of the eye disc in third instar larvae, and results in adults with normal eyes.

      (Kim and Choi, 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      CCT4NIG.5525R, Scer\GAL4ptc.PU has wing blade phenotype, enhanceable by Rheb2D1/Rheb[+]

      (Kim and Choi, 2019)
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      CCT4NIG.5525R is rescued by Scer\GAL4ptc.PU/CCT4UAS.cKa

      (Kim and Choi, 2019)

      CCT4NIG.5525R is rescued by Scer\GAL4ey.PU/CCT4UAS.cKa

      (Kim and Choi, 2019)

      CCT4NIG.5525R is rescued by Scer\GAL4nub.PU/CCT4UAS.cKa

      (Kim and Choi, 2019)
      Comments

      Co-expressing CCT4UAS.cKa with CCT4NIG.5525R under the control of Scer\GAL4ptc.PU rescues the size reduction of the wing blade domain between L3 and L4 veins, while co-expression under the control of Scer\GAL4nub.PU rescues wing formation, and co-expression under the control of Scer\GAL4ey.PU fully rescues head formation defects induced by CCT4NIG.5525R expression alone.

      (Kim and Choi, 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CCT4NIG.5525R
      CG5525NIG.5525R
      Name Synonyms
      Secondary FlyBase IDs
        References (4)