FB2025_01 , released February 20, 2025
Allele: Dmel\kluUAS.Tag:HA
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General Information
Symbol
Dmel\kluUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0276720
Feature type
allele
Associated gene
Dmel\klu
Associated Insertion(s)
Carried in Construct
P{UAS-HA-klu}
Also Known As
UAS-klu-HA
Key Links
Transgenic product class
wild_type
(Kaspar et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kaspar et al., 2008)
Mutagen
in vitro construct
(Kaspar et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-HA-klu}
Cytology
Description

UAS regulatory sequences drive expression of klu which is tagged at the N-terminal end with Tag:HA.

(Kaspar et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
klu
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of kluScer\UAS.T:Ivir\HA1 under the control of an insertion of Scer\GAL4GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2) results in supernumerary type II neuroblasts in larval brains. No supernumerary neuroblasts are seen when kluScer\UAS.T:Ivir\HA1 is expressed using an insertion of Scer\GAL4GMR9D11 that only expresses in ase[+] INPs (P{GMR9D11-GAL4}attP2).

      (Janssens et al., 2014)

      Clones derived from single type II neuroblasts expressing kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA mostly contain supernumerary neuroblasts.

      (Komori et al., 2014)

      Expression of kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA results in a massive expansion of type II neuroblasts in the brain, while the number of type I neuroblasts does not increase compared to wild type.

      Expression of kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP in type II neuroblast lineage clones induces supernumerary neuroblasts, while expression in type I neuroblast lineage clones does not.

      Expression of kluScer\UAS.T:Ivir\HA1 in immature intermediate neural progenitors under the control of Scer\GAL4erm.R9D11 results in the formation of supernumerary type II neuroblasts.

      (Xiao et al., 2012)

      Expression of kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4klu-G410 results in the formation of supernumerary bristles.

      (Kaspar et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      kluUAS.Tag:HA/Scer\GAL4GMR9D11 is a non-enhancer of abnormal neuroanatomy phenotype of bratDG19310/brat11

      (Janssens et al., 2014)
      Suppressor of
      Other
      Phenotype Manifest In
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of kluScer\UAS.T:Ivir\HA1 under the control of an insertion of Scer\GAL4GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2) enhances the supernumerary neuroblast phenotype seen in bratDG19310/brat11 mutant larval brains. No enhancement is seen when kluScer\UAS.T:Ivir\HA1 is expressed using an insertion of Scer\GAL4GMR9D11 that expresses in ase[+] INPs (P{GMR9D11-GAL4}attP2).

      Expression of kluScer\UAS.T:Ivir\HA1 under the control of an insertion of Scer\GAL4GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm1/Df(2L)erm2 mutants expressing ermT:Zzzz\FLAG).

      Expression of ermScer\UAS.T:Ivir\HA1 suppresses the supernumerary type II neuroblasts phenotype seen in larval brains when kluScer\UAS.T:Ivir\HA1 is expressed under the control of an insertion of Scer\GAL4GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2).

      In contrast to when either transgene is expressed alone, co-expression of ermzf.Scer\UAS.T:Hsim\VP16 and kluScer\UAS.T:Ivir\HA1 under the control of an insertion of Scer\GAL4GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) results in supernumerary type II neuroblasts in the larval brain.

      (Janssens et al., 2014)

      The formation of supernumerary neuroblasts which is seen in clones derived from single type II neuroblasts expressing kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA is partially suppressed if the clones are also mutant for arm8.

      The formation of supernumerary neuroblasts which is seen in clones derived from single type II neuroblasts expressing kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA is suppressed if the clones are also co-expressing Apc2Scer\UAS.T:Avic\GFP.

      (Komori et al., 2014)

      Co-expression of bratScer\UAS.T:Hsap\MYC suppresses the formation of supernumerary type II neuroblasts which is seen in type II neuroblast lineage clones expressing kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP.

      The premature loss of neuroblasts seen in N55e11 type II neuroblast clones is completely suppressed by expression of kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP. Supernumerary neuroblasts are not induced in these clones.

      Type I neuroblast clones in the larval brain co-expressing kluScer\UAS.T:Ivir\HA1 and Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP contain an average of 18 neuroblasts per clone and 100% of the clones show the supernumerary neuroblast phenotype.

      (Xiao et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      kluUAS.Tag:HA/Scer\GAL4klu-G410 rescues kluG410/klu212lR51C

      (Kaspar et al., 2008)
      Comments

      Expression of kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4klu-G410 rescues the bristle defects of klu212lR51C/kluG410 flies.

      (Kaspar et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      kluScer\UAS.T:Ivir\HA1
      kluUAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (5)