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General Information
Symbol
Dmel\smt3dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0276748
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-smt3i
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a smt3 RNAi construct.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of smt3dsRNA.Scer\UAS under the control of Scer\GAL4salm.EPv leads to a small but significant decrease in the total wing area, coupled with a severe area decrease of the intervein region between L2 and L3, as compared to controls; expression under the control of Scer\GAL4phm.PO leads to a complete development arrest at the larval stage.

The follicle cells in flies expressing smt3dsRNA.Scer\UAS under the control of Scer\GAL4T155 show a strong reduction in the number of lipid droplets. Egg fertility is only slightly affected and viable embryos are obtained.

The prothoracic gland cells in larvae expressing smt3dsRNA.Scer\UAS under the control of Scer\GAL4phm.PO have a reduced lipid content.

Expression of smt3dsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in a mutant phenotype in the eye.

Expression of smt3dsRNA.Scer\UAS under the control of Scer\GAL4ey.PU results in a dramatic reduction in the size of the eye disc and severely reduced adult eyes. In many cases, head formation is abolished and the animals do not eclose.

Animals expressing smt3dsRNA.Scer\UAS under the control of Scer\GAL4phm.PO arrest their development at the third larval instar just before pupariation and survive to an additional 3 weeks. During this time, the larvae continue feeding and gain weight. Mutant third instar larvae fed with medium containing 20-hydroxyecdysone pupariate within 24 hours, but do not develop further.

At 120-144 hours after egg laying (AEL), some prothoracic gland cells in larvae expressing smt3dsRNA.Scer\UAS under the control of Scer\GAL4phm.PO are increased in size. The nuclei of these cells are enlarged and show abnormal morphology. From 7 to 22 days AEL in the mutant larvae, the prothoracic gland cells and their nuclei continue to grow in size, and at the same time, the number of prothoracic gland cells progressively decreases. Massive cell death or degeneration are not seen in the mutant prothoracic glands. The cells show a decrease in the number of lipid droplets per cell compared to wild type.

Ultrastructurally, the prothoracic gland cells of larvae expressing smt3dsRNA.Scer\UAS under the control of Scer\GAL4phm.PO show a clear reduction in the number and length of invaginations of the plasma membrane compared to wild-type cells. The number, size and morphology of the mitochondria appear normal. The nuclear lamina appears thickened, but nuclear pores appear not to be affected.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Snmp1Scer\UAS.cBa partially rescues the reduction in the number of lipid droplets per cell in the prothoracic glands of 51% of larvae expressing smt3dsRNA.Scer\UAS under the control of Scer\GAL4phm.PO. The lipid droplets are comparable in size to controls. Expression of Snmp1Scer\UAS.cBa also rescues the lipid content in follicle cells expressing smt3dsRNA.Scer\UAS under the control of Scer\GAL4T155.

Xenogenetic Interactions
Statement
Reference

The decreases in area of the full wing and, in particular, of the intervein region between L2 and L3 resulting from the expression of smt3dsRNA.Scer\UAS under the control of Scer\GAL4salm.EPv, are enhanced, fully suppressed, or unaffected by co-expression of Hsap\SUMO3Scer\UAS.cUa, Bger\SUMO3Scer\UAS.cUa, or Bger\SUMO1Scer\UAS.cUa, respectively. The development arrest at the larval stage resulting from the expression of smt3dsRNA.Scer\UAS under the control of Scer\GAL4phm.PO is fully suppressed by the co-expression of Bger\SUMO1Scer\UAS.cUa, but not Bger\SUMO3Scer\UAS.cUa.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
smt3dsRNA.Scer\UAS
smt3dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)