Allele Dmel\orb2^F5Y

General Information
Symbol	Dmel\orb2^F5Y	Species	D. melanogaster
Name		FlyBase ID	FBal0276749
Feature type	allele	Associated gene	Dmel\orb2

Allele class
Mutagen	in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Majumdar et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference An 18.7kb wild-type genomic transgene that contains 560bp of the exons specific to the orb2 E-H isoforms and none of the I isoform. The phenylalanine at residue 5 has been replaced by a tyrosine. T:Avic\GFP[EGFP] is fused in frame in the C-terminal end. The construct contains a attB site. (Majumdar et al., 2012)
Carried in construct	P{orb2.F5Y} (Majumdar et al., 2012)
Tagged with	T:Avic\GFP^EGFP (Majumdar et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	orb2^F5Y partially rescues orb2^Δ (Majumdar et al., 2012)
Comments	Expression of orb2[F5Y] partially rescues the courtship behavior defects seen in orb2[Δ] mutant males. Memory in these flies begins to decay after 36 hour and there was no measurable memory by 48-60 hours. Expression of orb2[F5Y] partially rescues the defects in olfactory memory seen in orb2[Δ] mutant flies. No memory deficits are seen at 24 hours, but by 48 hours the flies have a lower memory score than wild-type flies. (Majumdar et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	orb2^F5Y
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Majumdar et al., 2012, Cell 148(3): 515--529 Critical Role of Amyloid-like Oligomers of Drosophila Orb2 in the Persistence of Memory. [FBrf0217407]

Allele Dmel\orb2F5Y

Allele Dmel\orb2^F5Y