Allele Dmel\ru^{Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\ru^{Scer\UAS.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0276764
Feature type	allele	Associated gene	Dmel\ru

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Yogev et al., 2010) in vitro construct - regulatory fusion (Yogev et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of ru tagged with T:Avic\GFP[EGFP]. The construct was made using a pUAST-attB vector. (Yogev et al., 2010)
Carried in construct	P{UAS-ru.eGFP} (Yogev et al., 2010)
Tagged with	T:Avic\GFP^EGFP (Yogev et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference ru^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^GMR.PF is a suppressor \| partially of visible \| adult stage phenotype of Rab11^{S25N.Scer\UAS}, Scer\GAL4^GMR.PF (Yogev et al., 2010) ru^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^GMR.PF is a suppressor \| partially of size defective \| adult stage phenotype of Rab11^{S25N.Scer\UAS} (Yogev et al., 2010)
Phenotype Manifest In
Suppressor of
Statement Reference ru^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^GMR.PF is a suppressor \| partially of eye phenotype of Rab11^{S25N.Scer\UAS} (Yogev et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Expression of ru[Scer\UAS.T:Avic\GFP-EGFP] partially rescues the small, rough eyes seen when Rab11[S25N.Scer\UAS] is expressed under the control of Scer\GAL4[GMR.PF]. (Yogev et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	ru^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^GMR.PF rescues ru^PLLb (Yogev et al., 2010) ru^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^MT14 rescues ru^PLLb (Yogev et al., 2010)
Partially rescues	ru^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^lz-gal4 partially rescues ru^PLLb (Yogev et al., 2010)
Fails to rescue	ru^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^hs.2sev fails to rescue ru^PLLb (Yogev et al., 2010) ru^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^sca-537.4 fails to rescue ru^PLLb (Yogev et al., 2010)
Comments	Expression of ru[Scer\UAS.T:Avic\GFP-EGFP] in all photoreceptors (R1 - R8) under the control of Scer\GAL4[GMR.PF] fully rescues the lamina cartridge neuron differentiation defects seen in the eye discs of ru[PLLb] mutant third instar larvae. Expression of ru[Scer\UAS.T:Avic\GFP-EGFP] in the R2, R5 and R8 photoreceptors under the control of Scer\GAL4[MT14] fully rescues the lamina cartridge neuron differentiation defects seen in the eye discs of ru[PLLb] mutant third instar larvae. Expression of ru[Scer\UAS.T:Avic\GFP-EGFP] in the R1, R6 and R7 photoreceptors under the control of Scer\GAL4[lz-gal4] partially rescues the lamina cartridge neuron differentiation defects seen in the eye discs of ru[PLLb] mutant third instar larvae. 20% of the wild type number of lamina cells express differentiation markers. Expression of ru[Scer\UAS.T:Avic\GFP-EGFP] in the R3, R4 and R7 photoreceptors under the control of Scer\GAL4[hs.2sev] fails to rescue the lamina cartridge neuron differentiation defects seen in the eye discs of ru[PLLb] mutant third instar larvae. Expression of ru[Scer\UAS.T:Avic\GFP-EGFP] in the R4 and weakly in the R3 photoreceptors under the control of Scer\GAL4[hs.2sev] fails to rescue the lamina cartridge neuron differentiation defects seen in the eye discs of ru[PLLb] mutant third instar larvae. Expression of ru[Scer\UAS.T:Avic\GFP-EGFP] in the R8 photoreceptors under the control of Scer\GAL4[sca-537.4] fails to rescue the lamina cartridge neuron differentiation defects seen in the eye discs of ru[PLLb] mutant third instar larvae. (Yogev et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ru^{Scer\UAS.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Yogev et al., 2010, PLoS Biol. 8(10): e1000505 Polarized Secretion of Drosophila EGFR Ligand from Photoreceptor Neurons Is Controlled by ER Localization of the Ligand-Processing Machinery. [FBrf0212078]

Allele Dmel\ruScer\UAS.T:Avic\GFP-EGFP

Allele Dmel\ru^{Scer\UAS.T:Avic\GFP-EGFP}