Allele Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}

General Information
Symbol	Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}	Species	a. artificial
Name		FlyBase ID	FBal0276808
Feature type	allele	Associated gene	Zzzz\CAG

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (McLeod et al., 2005) in vitro construct - regulatory fusion (McLeod et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a poly-glutamine-repeat encode by 94 CAA codons fused with C-terminal T:Hsap\MYC and T:Zzzz\FLAG tags. (McLeod et al., 2005)
Carried in construct	P{UAS-CAA.94} (McLeod et al., 2005, Lawlor et al., 2012, van Eyk et al., 2011)
Tagged with	T:Hsap\MYC (McLeod et al., 2005) T:Zzzz\FLAG (McLeod et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^GMR.PF (McLeod et al., 2005) viable, with Scer\GAL4^da.G32 (Lawlor et al., 2012) visible, with Scer\GAL4^GMR.PF (McLeod et al., 2005) visible, with Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (McLeod et al., 2005) eye, with Scer\GAL4^GMR.PFa (van Eyk et al., 2011) ommatidium, with Scer\GAL4^GMR.PF (McLeod et al., 2005) retinal pigment cell, with Scer\GAL4^GMR.PF (McLeod et al., 2005)
Detailed Description
	Statement Reference The tergites of flies expressing Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[da.G32] appear normal compared to wild-type. (Lawlor et al., 2012) Expression of Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] results in pigmentation loss in the eye. (van Eyk et al., 2011) Expression of Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] in the developing eye under the control of Scer\GAL4[GMR.PF] causes a dramatic phenotype with disorganization of the ommatidia, loss of pigment cells and a collapsed eye. Depending on the insertion line used, expression of Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] results in phenotypes ranging in severity from wild-type to lethality. (McLeod et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, enhanceable by mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, enhanceable by sgg^Scer\UAS.cBa/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-enhanceable by Mef2^GD5039, Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-enhanceable by sgg^KK108994/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-suppressible by Mef2^GD5039, Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-suppressible by sgg^KK108994/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, enhanceable by mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, enhanceable by sgg^Scer\UAS.cBa/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-enhanceable by Mef2^GD5039, Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-enhanceable by sgg^KK108994/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-suppressible by Mef2^GD5039, Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-suppressible by sgg^KK108994/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Mef2[GD5039] with Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] does not alter the appearance of the eye. Co-expression of mod(mdg4)[GD16547] with Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] results in a mild enhancement of the Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG]-induced eye pigmentation defects. Co-expression of sgg[Scer\UAS.cBa] with Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] results in an increase in the eye compared with ectopic expression of sgg[Scer\UAS.cBa] alone. There appears to be a reduction in the amount of pigment in the eye and in most cases there are necrotic patches and nearly complete loss of ommatidial array structure. Co-expression of sgg[KK108994] with Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] does not dramatically alter the exterior appearance of the eye compared with flies expressing Zzzz\CAG[CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] alone. (van Eyk et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} Zzzz\CAG^{CAA.94.T:Hsap\MYC,T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Lawlor et al., 2012, PLoS ONE 7(6): e38516 Ubiquitous Expression of CUG or CAG Trinucleotide Repeat RNA Causes Common Morphological Defects in a Drosophila Model of RNA-Mediated Pathology. [FBrf0218612] van Eyk et al., 2011, Hum. Mol. Genet. 20(14): 2783--2794 Perturbation of the Akt/Gsk3-{beta} signalling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs. [FBrf0213942] McLeod et al., 2005, Hum. Mol. Genet. 14(8): 1041--1048 The pathogenic agent in Drosophila models of 'polyglutamine' diseases. [FBrf0187723]

Allele Zzzz\CAGCAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG

Allele Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}