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General Information
Symbol
Zzzz\Poly-AlaA37.UAS.EGFP,Tag:NLS(SV40-largeT)
Species
a. artificial
Name
FlyBase ID
FBal0276825
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-NLS-A37
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt drives expression of 37 polyalanine repeats that are tagged with EGFP and a nuclear localization signal (Tag:NLS(SV40-largeT)).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2 under the control of Scer\GAL4ey.PB causes toxicity resulting in survival to adulthood and eye abnormalities. Survival of the mutants is increased by rapamycin treatment. Rapamycin also decreases the toxicity of Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2 in the eye.

The expression of Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls-largeT under the control of Scer\GAL4nrv2.PS, Scer\GAL4ey.PB or Scer\GAL4Mhc.PW lead to partially lethality before adulthood. Adult survivors expressing Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls-largeT under the control of Scer\GAL4nrv2.PS present a small decrease in lifespan as compared to controls. Adult survivors expressing Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls-largeT under the control of Scer\GAL4ey.PB exhibit a rough and small eye phenotypes, as compared to controls.

Expression of Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2 under the control of Scer\GAL4ey.PB results in a rough eye phenotype which can be partially rescued by Li[+] or by AR-A014418.

The semi-lethality caused by expression of Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2 under the control of Scer\GAL4ey.PB can be partially rescued by AR-A014418.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of panScer\UAS.cWa decreases the fraction of flies with rough eyes in animals expressing Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2 under the control of Scer\GAL4ey.PB.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Avic\GFPA37.EGFP.Scer\UAS.T:Zzzz\Poly-Ala.T:SV40\nls2
Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls-largeT
Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2
Zzzz\Poly-AlaA37.UAS.EGFP,Tag:NLS(SV40-largeT)
Name Synonyms
Secondary FlyBase IDs
  • FBal0276692
  • FBal0265630
References (4)