Allele Hsap\APP^{T22.Aβ42.Scer\UAS}

General Information
Symbol	Hsap\APP^{T22.Aβ42.Scer\UAS}	Species	H. sapiens
Name		FlyBase ID	FBal0276874
Feature type	allele	Associated gene	Hsap\APP

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Speretta et al., 2012) in vitro construct - regulatory fusion (Speretta et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a tandem construct containing two copies of the Aβ (amyloid β) monomer linked together by a 22 amino acid linker peptide. Both copies of Aβ are the 42 amino acid residue form. (Speretta et al., 2012)
Carried in construct	M{UAS-T22-Aβ42} (Speretta et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	melanotic necrosis \| adult stage, with Scer\GAL4^GMR.PF (Speretta et al., 2012) short lived, with Scer\GAL4^elav-C155 (Speretta et al., 2012) visible, with Scer\GAL4^GMR.PF (Speretta et al., 2012)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Speretta et al., 2012)
Detailed Description
	Statement Reference Flies expressing Hsap\APP[T22.Aβ42.Scer\UAS] under the control of Scer\GAL4[GMR.PF] form significant quantities of large, SDS-insoluble deposits in the head. Flies expressing Hsap\APP[T22.Aβ42.Scer\UAS] under the control of Scer\GAL4[GMR.PF] have severe eye malformation with loss of the ommatidial arrays and the appearance of melanised patches of necrotic tissue. Flies expressing one copy of Hsap\APP[T22.Aβ42.Scer\UAS] under the control of Scer\GAL4[elav-C155] have a significantly shorter lifespan than wild-type controls and than flies expressing two copies of Hsap\APP[Aβ42.Scer\UAS.att] under the control of Scer\GAL4[elav-C155]. (Speretta et al., 2012)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\APP^{T22.Aβ42.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Speretta et al., 2012, J. Biol. Chem. 287(24): 20748--20754 Expression in Drosophila of Tandem Amyloid β Peptides Provides Insights into Links between Aggregation and Neurotoxicity. [FBrf0218555]

Allele Hsap\APPT22.Aβ42.Scer\UAS

Allele Hsap\APP^{T22.Aβ42.Scer\UAS}