A 3844 base pair fragment from the flanking non-coding or intronic region of Vsx2 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Ecol\lexAGMR71G01 drives expression in a subset of neurons in the adult CNS, including the male-specific P1 (pMP-e) neurons in the brain. The P1 neurons overlap with the expression of Scer\GAL4fru.P1.B or Scer\GAL4dsx-GAL4 in males. A small number of abdominal ganglion neurons and glial cells are also detected when expressing both Ecol\lexAGMR71G01 and Scer\GAL4dsx-GAL4.