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General Information
Symbol
Scer\GAL4dsx.KI
Species
S. cerevisiae
Name
FlyBase ID
FBal0277019
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dsx-GAL4, dsxGal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Sequence encoding a GAL4 driver has been inserted into the first, non-sex specific coding exon of dsx. The resulting allele produces wild-type dsx transcripts (represented by dsxKI.GAL4) and GAL4-containing transcripts (represented by Scer\GAL4dsx.KI) in both sexes.

Insertion components
TI{GAL4}dsxKI.GAL4
Product class / Tool use(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4dsx.KI drives expression in four of twelve neuroblasts in the larval terminal abdominal neuromere; in female larvae, these neuroblasts die during mid-third instar.

Drives expression in doublesex-expressing neurons and neuroblasts, and in oenocytes, at all developmental stages.

Scer\GAL4dsx.KI drives expression in non-neuronal tissues, including unidentified sub- and peri-cuticular cells.

Scer\GAL4dsx.KI drives expression in both male and female polyploid enterocytes, but not in esg-positive precursors, or enteroendocrine cells.

Scer\GAL4dsx.KI drives expression in the posterior part of the thoracico-abdominal ganglion and a subset of neurons in the adult brain. In males expression in the brain is stronger than in females and additional expression in more anterior parts of the thoracico-abdominal ganglion are detected.

Scer\GAL4dsx.KI drives expression in 3 clusters of neurons in the adult female genital tract: one cluster of 1 neuron in the lateral oviduct (which co-localises with Avic\GFPEGFP.ppk.1) and two clusters in the uterus. An anterior one with 3 neurons (which also express Avic\GFPEGFP.ppk.1) and one more posterior with 7 neurons which co-express Avic\GFPEGFP.ppk.1, with 3 neurons of those also expressing Ecol\lexAfru.P1.T:Hsim\VP16. In combination with Scer\FLP1250, Scer\GAL4dsx.KI drives expression in 27, out of a total of 300 neurons, in the abdominal ganglion of adult females. These neurons include four ascending abdominal ganglion neurons that terminate in the subesophageal ganglion, and two subesophageal ganglion descending neurons that innervate the uterus. Sex specific differences were observed, with the majority of males displaying only one ascending neuron, and half of the males displaying two descending neurons.

Scer\GAL4dsx.KI drives expression in several cell clusters in the CNS throughout development, with some of the cell clusters being either male specific (SN, TN1 and TN2), or composed of more cells in males. In the adult CNS, expression is observed in 900 neurons in males (9 clusters) and 700 neurons in females (6 clusters). Co-localisation with fru protein is observed in some clusters including pC1, pC2, pC3 and in the abdominal ganglion.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Blocking synaptic vesicle exocytosis (using Ctet\TeTxLCTNT.Scer\UAS) in Scer\GAL4dsx.KI neurons in males and females results in a significant drop (compared to genetic controls) in courtship of females by males, males completely fail to copulate and are behaviorally sterile; females are sterile, unreceptive and show no post-mating behavioral responses.

Thermogenetic activation (using TrpA1UAS.cUa at 32[o]C) of Scer\GAL4dsx.KI cells in mature adult virgin or mated females induces horizontal (mating-type) ovipositor extrusion and oviposition behaviors that are not induced in controls; oviposition behaviors, but not horizontal ovipositor extrusion, still occur in mated females when activation is further restricted using Scer\FLP1FLPo.oc.Tag:NLS(SV40-largeT) and Scer\GAL80FRT.αTub84B.

Thermogenetic activation (using TrpA1FRT.stop.UAS.Tag:MYC at 32[o]C) of a Scer\FLP1FLPo.oc.Tag:NLS(SV40-largeT)-expressing subset of Scer\GAL4dsx.KI cells in adult mated females induces horizontal ovipositor extrusion, but not oviposition behaviors, that are not induced in controls.

Blocking cell death (using BacA\p35UAS.cHa) in Scer\GAL4dsx.KI cells in males results in ectopic formation of usually female-specific pMN2 neurons; pMN2 neurons are still present in females of this genotype as normal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (28)