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General Information
Symbol
Hsap\KCNJ2UAS.GFP
Species
H. sapiens
Name
FlyBase ID
FBal0277023
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The FRT cassette has been excised, allowing expression of Kir2.1 fused to GFP under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\KCNJ2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ppk.PU in class IV dendritic arborization (da) neuron clones inhibits both spontaneous and light-evoked activity in these neurons.

Expression of Hsap\KCNJ2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ppk.PU in single cell class IV da neuron clones results in a shift in the presynaptic terminals in v'ada neurons

Single v'ada class IV da neuron cells expressing Hsap\KCNJ2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ppk.PU (clones induced in the early first or second larval instar stages) show a ventral shift in the position of their presynaptic terminals compared to controls. The topography of the presynaptic terminals of ddaC or vdaB class IV da neurons is unaffected if they express Hsap\KCNJ2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ppk.PU as single cell clones.

Silencing of neurons expressing both 5-HT1B and fru (through expression of Hsap\KCNJ2Scer\UAS.T:Avic\GFP under the control of Scer\GAL45-HT1B.PY and in which the Scer\FRT stop cassette has been removed by Scer\FLP1fru) impairs the inhibition of female contact-induced male-male aggression seen in wild type males that have previously been exposed to females.

Silencing of neurons expressing both Rdl and fru (through expression of Hsap\KCNJ2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Rdl.PS and in which the Scer\FRT stop cassette has been removed by Scer\FLP1fru) results in lethality or severe courtship issues.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The dorsal shift of presynaptic terminals compared to controls which is seen in single Trim9unspecified vdaB class IV dendritic arborization neuron cells is still seen if they are expressing Hsap\KCNJ2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ppk.PU.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\KCNJ2Scer\UAS.T:Avic\GFP
Hsap\KCNJ2UAS.GFP
Name Synonyms
Secondary FlyBase IDs
    References (4)