Allele Dmel\Smn^{T205I.T:Zzzz\FLAG}

General Information
Symbol	Dmel\Smn^{T205I.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0277064
Feature type	allele	Associated gene	Dmel\Smn

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Praveen et al., 2012) in vitro construct - coding region fusion (Praveen et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A ~3kb fragment containing the entire Smn coding region is tagged with T:Zzzz\FLAG. The threonine at amino acid 205 has been replaced with isoleucine. (Praveen et al., 2012)
Carried in construct	P{Smn.T205I.FLAG} (Praveen et al., 2012)
Tagged with	T:Zzzz\FLAG (Praveen et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective, with Smn^X7/Smn^f01109 (Praveen et al., 2012) partially lethal - majority die \| pupal stage, with Smn^X7/Smn^f01109 (Praveen et al., 2012)
Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Smn^{T205I.T:Zzzz\FLAG} partially rescues Smn^X7/Smn^f01109 (Praveen et al., 2012)
Comments	Expression of Smn[T205I.T:Zzzz\FLAG] fully rescues the larval lethality seen in Smn[f01109]/Smn[X7] mutants. However lethality in these mutants occurs at the pupal stage, with only 25% of mutants eclosing as adults. Pupation and eclosion occurs a day later than in wild type controls. Expression of Smn[T:Zzzz\FLAG] also largely rescues the motility defects seen in Smn[f01109]/Smn[X7] mutant larvae, whereas the burrowing defects are fully rescued. (Praveen et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Smn^{T205I.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Praveen et al., 2012, Cell Rep. 1(6): 624--631 A Drosophila Model of Spinal Muscular Atrophy Uncouples snRNP Biogenesis Functions of Survival Motor Neuron from Locomotion and Viability Defects. [FBrf0218971]

Allele Dmel\SmnT205I.T:Zzzz\FLAG

Allele Dmel\Smn^{T205I.T:Zzzz\FLAG}