Allele Dmel\ple^{dsRNA.Sym.Scer\UAS}

General Information
Symbol	Dmel\ple^{dsRNA.Sym.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0277073
Feature type	allele	Associated gene	Dmel\ple

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Neckameyer and Bhatt, 2012) in vitro construct - RNAi (Neckameyer and Bhatt, 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences positioned at either end of the coding region drive expression of full-length ple coding sequences, in both sense and antisense orientation, resulting in the generation of dsRNA. (Neckameyer and Bhatt, 2012)
Carried in construct	P{UAS-ple.dsRNA} (Neckameyer and Bhatt, 2012)
Cytology
Phenotypic Data
Phenotypic Class
	feeding behavior defective \| larval stage, with Scer\GAL4^elav-C155 (Neckameyer and Bhatt, 2012) feeding behavior defective \| larval stage, with Scer\GAL4^ple.PF (Neckameyer and Bhatt, 2012)
Phenotype Manifest In
	serotonergic neuron, with Scer\GAL4^elav-C155 (Neckameyer and Bhatt, 2012) serotonergic neuron, with Scer\GAL4^ple.PF (Neckameyer and Bhatt, 2012)
Detailed Description
	Statement Reference A reduction in ple protein levels, brought about through expression of one or two copies of ple[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[elav-C155], correlates with a reduction in larval feeding, despite the larvae appearing able to feed. While not significant, there is a trend towards increased branching of the 5-HT axonal fibers. However, the number and size of the 5-HT-containing vesicles are significantly increased with increasing knockdown of ple. Overall, the reduction in ple levels correlates with increased vesicle area. varicosity number is also increased. Exogenous dopamine exposure during the last 6 hours of embryogenesis can rescue the feeding behavior defects and fiber branching, total varicosity number, the number of varicosities greater than 5υm[2], and varicosity area found in Scer\GAL4[elav-C155] ple[dsRNA.Sym.Scer\UAS]/ple[dsRNA.Sym.Scer\UAS] mutants. There is no change in pupal size for these mutants and no change in time to pupariation, but there is a direct correlation between the extent of knockdown and the number of pupae. Expression of ple[dsRNA.Sym.Scer\UAS] in 2nd instar larvae, under the control of Scer\GAL4[elav.Switch.PO] does not affect feeding behaviour, locomotion or gut fiber architecture. Branching, varicosity number, varicosity area and the number of large varicosities are unaffected. Expression of ple[dsRNA.Sym.Scer\UAS] in serotonergic neurons (under the control of Scer\GAL4[l.Trh.PK]) does not affect feeding behaviour, while expression in DA neurons, under the control of Scer\GAL4[ple.PF]) reduces feeding. Similarly, increased gut fiber branching and increased numbers of large 5-HT-containing vesicles are seen upon expression in DA neurons, but not upon expression in serotonergic neurons. (Neckameyer and Bhatt, 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^elav-C155, ple^{dsRNA.Sym.Scer\UAS} has feeding behavior defective \| larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Trh^{dsRNA.Sym.Scer\UAS} (Neckameyer and Bhatt, 2012) Scer\GAL4^ple.PF, ple^{dsRNA.Sym.Scer\UAS} has feeding behavior defective \| larval stage phenotype, suppressible by Trh^{dsRNA.Sym.Scer\UAS}/Scer\GAL4^l.Trh.PK (Neckameyer and Bhatt, 2012)
Suppressor of
Statement Reference ple^{dsRNA.Sym.Scer\UAS}/Scer\GAL4^ple.PF is a suppressor of feeding behavior defective \| larval stage phenotype of Scer\GAL4^ple.PF, Trh^{dsRNA.Sym.Scer\UAS} (Neckameyer and Bhatt, 2012) Scer\GAL4^elav-C155/ple^{dsRNA.Sym.Scer\UAS} is a suppressor of feeding behavior defective \| larval stage phenotype of Scer\GAL4^elav-C155, Trh^{dsRNA.Sym.Scer\UAS} (Neckameyer and Bhatt, 2012)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^elav-C155, ple^{dsRNA.Sym.Scer\UAS} has serotonergic neuron phenotype, suppressible by Scer\GAL4^elav-C155/Trh^{dsRNA.Sym.Scer\UAS} (Neckameyer and Bhatt, 2012) Scer\GAL4^ple.PF, ple^{dsRNA.Sym.Scer\UAS} has serotonergic neuron phenotype, suppressible by Trh^{dsRNA.Sym.Scer\UAS}/Scer\GAL4^l.Trh.PK (Neckameyer and Bhatt, 2012)
Suppressor of
Statement Reference ple^{dsRNA.Sym.Scer\UAS}/Scer\GAL4^ple.PF is a suppressor of serotonergic neuron phenotype of Scer\GAL4^elav-C155, Trh^{dsRNA.Sym.Scer\UAS} (Neckameyer and Bhatt, 2012) ple^{dsRNA.Sym.Scer\UAS}/Scer\GAL4^ple.PF is a suppressor of serotonergic neuron phenotype of Scer\GAL4^ple.PF, Trh^{dsRNA.Sym.Scer\UAS} (Neckameyer and Bhatt, 2012)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of ple[dsRNA.Sym.Scer\UAS] and Trh[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[elav-C155], does not affect larval feeding, unlike expression of either single transgene. When the same combination is placed under the control of either Scer\GAL4[ple.PF] or Scer\GAL4[l.Trh.PK], feeding is reduced, consistent with knockdown of only DA or 5-HT synthesis, but not both. Gut fiber architecture is rescued to control levels by the presence of both ple[dsRNA.Sym.Scer\UAS] and Trh[dsRNA.Sym.Scer\UAS]. (Neckameyer and Bhatt, 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ple^{dsRNA.Sym.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Neckameyer and Bhatt, 2012, BMC Neurosci. 13: 26 Neurotrophic actions of dopamine on the development of a serotonergic feeding circuit in Drosophila melanogaster. [FBrf0218484]

Allele Dmel\pledsRNA.Sym.Scer\UAS

Allele Dmel\ple^{dsRNA.Sym.Scer\UAS}