Allele Dmel\ple^Scer\UAS.cNa

General Information
Symbol	Dmel\ple^Scer\UAS.cNa	Species	D. melanogaster
Name		FlyBase ID	FBal0277074
Feature type	allele	Associated gene	Dmel\ple

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Neckameyer and Bhatt, 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of full-length ple coding sequences. (Neckameyer and Bhatt, 2012)
Carried in construct	P{UAS-ple.N} (Neckameyer and Bhatt, 2012)
Cytology
Phenotypic Data
Phenotypic Class
	feeding behavior defective \| larval stage, with Scer\GAL4^elav-C155 (Neckameyer and Bhatt, 2012)
Phenotype Manifest In
	serotonergic neuron, with Scer\GAL4^elav-C155 (Neckameyer and Bhatt, 2012)
Detailed Description
	Statement Reference Expression of ple[Scer\UAS.cNa] under the control of Scer\GAL4[elav-C155] during late embryogenesis results in depressed feeding behavior in larvae. There is no change in pupal size for these mutants and no change in time to pupariation, but there is a direct correlation between the extent of knockdown and the number of pupae. Locomotor behavior is normal in these flies. The 5-HT axonal fibers projecting to the gut from the larvae, with increased neuronal DA levels due to ple[Scer\UAS.cNa] expression, display greater complexity relative to controls: increased fiber branching, increased number of 5-HT-containing vesicles along the axon length, and greater numbers of larger vesicles. There is no change in pupal size for these mutants and no change in time to pupariation, but there is a direct correlation between the extent of overexpression and the number of pupae. Expression of ple[Scer\UAS.cNa] in 2nd instar larvae, under the control of Scer\GAL4[elav.Switch.PO] does not affect feeding behavior, locomotion or gut fiber architecture. Branching, varicosity number, varicosity area and the number of large varicosities are unaffected. (Neckameyer and Bhatt, 2012)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ple^Scer\UAS.cNa
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Neckameyer and Bhatt, 2012, BMC Neurosci. 13: 26 Neurotrophic actions of dopamine on the development of a serotonergic feeding circuit in Drosophila melanogaster. [FBrf0218484]

Allele Dmel\pleScer\UAS.cNa

Allele Dmel\ple^Scer\UAS.cNa