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General Information
Symbol
Dmel\Claspin45
Species
D. melanogaster
Name
FlyBase ID
FBal0277228
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:

Deletion resulting from the imprecise excision of P{EP}GE20270, extending from -2347 to +5195, where +1 is the translation start of Claspin. A portion of the EP element remains.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a deletion which removes the entire coding sequence of Claspin. The deletion extends to -2347bp relative to the Claspin transcription start site. Part of the original inserted P{EP} element remains.

Insertion components
P{?EP}Claspin45
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eggs laid by homozygous females show a very low rate of hatching (0.2%). Eggs laid by Claspin45/Df(3L)Exel8098 females fail to hatch. Most embryos laid by the mutant females show normal nuclear morphology until division 10, but after this stage they begin to show an irregular distribution of surface nuclei and and have nuclei with abnormal size or shape. By division 13, less than one-third of the mutant embryos have normal nuclear morphology.

Homozygous and Claspin45/Df(3L)Exel8098 animals show an increased frequency of generation of mutant clones compared to wild type in a loss of heterozygosity assay.

Ionising radiation-induced G2 arrest occurs normally in homozygous eye discs. Ionising radiation-induced apoptosis occurs normally in homozygous eye discs.

Homozygous and Claspin45/Df(3L)Exel8098 eye discs show defects in hydroxyurea-induced G2 arrest.

Homozygous and Claspin45/Df(3L)Exel8098 larvae show reduced survival in the presence of hydroxyurea compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)