Homozygous, hemizygous, or heteroallelic CRMPsupIa1 mutants are viable, fertile, and morphologically normal when grown under standard laboratory conditions.
When tested for short term memory performance 2 minutes after a single aversive Pavlovian training session, CRMPsupK1/CRMPsupIa1 as well as homozygous CRMPsupIa1 mutants exhibit significantly reduced performance compared with wild-type control flies. Medium-term memory performance measured 3 hours after a single aversive Pavlovian training session is also reduced in homozygous mutants and heteroallelic mutant animals.
Under free-running conditions (constant darkness), wild-type and CRMPsupIa1 mutant animals exhibit similar overall activity. Period length is similar for all tested animals, indicating that the length of the circadian clock is not affected by CRMPsupIa1. Compared with wild-type and heterozygous animals, significantly reduced arrhythmic power values indicating the relative strength of rhythmicity of free-running behaviours are observed among CRMPsupIa1/Df(3R)noi-B hemizygotes and CRMPsupK1/CRMPsupIa1 heteroallelic animals. Surprisingly, power values for CRMPsupIa1 homozygotes are not significantly reduced.