Allele Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}

General Information
Symbol	Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}	Species	a. artificial
Name		FlyBase ID	FBal0277239
Feature type	allele	Associated gene	Zzzz\CAG

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Lawlor et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an approximately 100 nt CAG repeat in reverse orientation. (Lawlor et al., 2011)
Carried in construct	P{UAS-rCUG.100} (Lawlor et al., 2011, Lawlor et al., 2012)
Tagged with	T:Hsap\MYC (Lawlor et al., 2011) T:Zzzz\FLAG (Lawlor et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| heat sensitive, with Scer\GAL4^da.G32 (Lawlor et al., 2012) lethal \| male limited, with Scer\GAL4^elav-C155, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2011) locomotor behavior defective, with Scer\GAL4^elav.PLu, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2011) visible, with Scer\GAL4^da.G32 (Lawlor et al., 2012) visible, with Scer\GAL4^elav-C155, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2011) visible, with Scer\GAL4^GMR.PF, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2011)
Phenotype Manifest In
	eye, with Scer\GAL4^elav-C155, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2011) eye, with Scer\GAL4^GMR.PF, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2011) tergite, with Scer\GAL4^Act5C.PI (Lawlor et al., 2012) tergite, with Scer\GAL4^da.G32 (Lawlor et al., 2012)
Detailed Description
	Statement Reference At the standard culture temperature of 25[o]C, a significant reduction in viability is observed for one of two independent Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG]-expressing lines using Scer\GAL4[da.G32] compared with empty vector controls. When grown at 29[o]C, complete lethality is observed in Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG]-expressing progeny but not in empty vector controls using Scer\GAL4[da.G32]. Closer physical examination of adult flies reveals a phenotype in viable progeny expressing Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] using Scer\GAL4[da.G32] at 25[o]C whereby the tergites of the dorsal abdomen are not correctly formed. In wild-type adults, the abdomen contains a series of regularly arranged tergites, while in Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG]-expressing flies one or more of the tergite bands is split down the midline so that the two sides do not meet at all, or meet only partially. Expression of Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] in histoblasts using Scer\GAL4[T155] does not result in significant tergite abnormalities. (Lawlor et al., 2012) Flies expressing Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] alone under the control of Scer\GAL4[elav-C155] display normal eye patterning. Co-expression of Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] and Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] results in a rough eye phenotype. Pan-neural co-expression of Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] and Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] using Scer\GAL4[elav-C155] results in a significant level of male lethality, while females show a mild phenotype in the adult eye. (Lawlor et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^elav-C155, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, enhanceable by Dcr-2^Scer\UAS.cDa, Scer\GAL4^elav-C155 (Lawlor et al., 2011) Scer\GAL4^GMR.PF, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, enhanceable by Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Lawlor et al., 2011)
NOT Enhanced by
Statement Reference Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-enhanceable by Dcr-2[+]/Dcr-2^L811fsX (Lawlor et al., 2012)
Suppressed by
Statement Reference Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, suppressible \| partially by Dcr-1^Q1147X/Dcr-1[+] (Lawlor et al., 2012) Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, suppressible \| partially by mbl[+]/mbl^E27 (Lawlor et al., 2012) Scer\GAL4^GMR.PF, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, suppressible \| partially by Dcr-2[+]/Dcr-2^L811fsX (Lawlor et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-suppressible by Dcr-2[+]/Dcr-2^L811fsX (Lawlor et al., 2012)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^elav-C155, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, enhanceable by Dcr-2^Scer\UAS.cDa, Scer\GAL4^elav-C155 (Lawlor et al., 2011) Scer\GAL4^GMR.PF, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, enhanceable by Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Lawlor et al., 2011)
NOT Enhanced by
Statement Reference Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has tergite phenotype, non-enhanceable by Dcr-2[+]/Dcr-2^L811fsX (Lawlor et al., 2012)
Suppressed by
Statement Reference Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has tergite phenotype, suppressible \| partially by Dcr-1^Q1147X/Dcr-1[+] (Lawlor et al., 2012) Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has tergite phenotype, suppressible \| partially by mbl[+]/mbl^E27 (Lawlor et al., 2012) Scer\GAL4^GMR.PF, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, suppressible \| partially by Dcr-2[+]/Dcr-2^L811fsX (Lawlor et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has tergite phenotype, non-suppressible by Dcr-2[+]/Dcr-2^L811fsX (Lawlor et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Statistical analysis reveals a significant decrease in the proportion of flies expressing Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] using Scer\GAL4[da.G32] that show any phenotype in the presence of heterozygous mbl[E27] compared with flies expressing Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] alone. However, no change is observed when comparing the proportion within the strongest two categories only, perhaps indicating that only those with the weakest phenotype are suppressed. Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] expression using Scer\GAL4[da.G32] in the presence of heterozygous Dcr-2[L811fsX] does not give significantly different phenotype severity compared to Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] expression in a wild-type Dcr-2 background. In one of two lines expressing Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] using Scer\GAL4[da.G32] in the presence of heterozygous Dcr-1[Q1147X] the proportion of progeny with any tergite-disruption phenotype, and those with a strong phenotype, is significantly reduced compared with flies expressing Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] alone. (Lawlor et al., 2012) Heterozygous Dcr-2[L811fsX] suppresses the eye-phenotype resulting from the co-expression of Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] and Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF]. In addition, the overall disruption observed in patterning of the internal structure of the eye is significantly reduced in the presence of heterozygous Dcr-2[L811fsX] as revealed by sections through adult eyes. Co-expression of Dcr-2[Scer\UAS.cDa] with Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG]/Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] at 18[o]C results in a loss-of-pigment phenotype which is not observed when Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG]/Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] is expressed under the control of Scer\GAL4[GMR.PF]. The normal eye phenotype of flies expressing Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] is not modified by the co-expression of Dcr-2[Scer\UAS.cDa]. The eye phenotype resulting from the co-expression of Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] and Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] using Scer\GAL4[elav-C155] is significantly enhanced when Dcr-2[Scer\UAS.cDa] is also co-expressed. Co-expression of Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] and Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[elav.PLu] fails to induce defects in the adult eye or viability, however, flies show an age-dependent movement disorder (failure-to-climb phenotype) compared with controls. (Lawlor et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Lawlor et al., 2012, PLoS ONE 7(6): e38516 Ubiquitous Expression of CUG or CAG Trinucleotide Repeat RNA Causes Common Morphological Defects in a Drosophila Model of RNA-Mediated Pathology. [FBrf0218612] Lawlor et al., 2011, Hum. Mol. Genet. 20(19): 3757--3768 Double-stranded RNA is pathogenic in Drosophila models of expanded repeat neurodegenerative diseases. [FBrf0215240]

Allele Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG

Allele Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}