Allele Dmel\psidin^55D4

General Information
Symbol	Dmel\psidin^55D4	Species	D. melanogaster
Name		FlyBase ID	FBal0277250
Feature type	allele	Associated gene	Dmel\psidin

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms neuroanatomy defective adult olfactory receptor neuron Or33c/85e adult olfactory receptor neuron Or42a adult olfactory receptor neuron Or59c adult olfactory receptor neuron Or67d lamellipodium adult stage adult olfactory receptor neuron Or22
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Kim et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: K471@. (Stephan et al., 2012) Amino acid replacement: ?471@. (Kim et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (Kim et al., 2011) lethal (with psidin^85D1) (Kim et al., 2011) lethal (with psidin^e02846) (Kim et al., 2011) neuroanatomy defective \| adult stage \| somatic clone (Stephan et al., 2012)
Phenotype Manifest In
	adult olfactory receptor neuron Or22 \| somatic clone (Stephan et al., 2012) adult olfactory receptor neuron Or33c/85e \| somatic clone (Stephan et al., 2012) adult olfactory receptor neuron Or42a \| somatic clone (Stephan et al., 2012) adult olfactory receptor neuron Or59c \| somatic clone (Stephan et al., 2012) adult olfactory receptor neuron Or67d \| somatic clone (Stephan et al., 2012) border follicle cell (Kim et al., 2011) border follicle cell \| somatic clone (Kim et al., 2011) lamellipodium (Stephan et al., 2012)
Detailed Description
	Statement Reference Homozygous olfactory receptor neuron (ORN) clones result in complete loss of the Or33c and Or42a-expressing ORNs and a mild reduction in cell number of the Or67d, Or22a and Or59c ORNs. Homozygous ORN clones result in defects in glomerulus targeting. Mutant Or59c ORNs show a strong mistargeting phenotype. Neurons in primary neuron cultures derived from mutant embryos have significantly smaller lamellipodia and significantly longer neurites compared to wild-type controls. (Stephan et al., 2012) Homozygous border cells show defects in migration: many of the mutant cells show no migration at all, and more than 80% of mutant clusters fail to reach the oocyte by stage 10. Heterozygous border follicle cells have shorter actin-rich protrusions than controls. (Kim et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference psidin^55D4 has neuroanatomy defective \| somatic clone \| adult stage phenotype, suppressible \| partially \| somatic clone by Tm1^ZCL0722 (Stephan et al., 2012)
Other
Statement Reference psidin^55D4/psidin[+], tsr¹/tsr^ntf has lethal phenotype (Kim et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference psidin^55D4 has adult olfactory receptor neuron Or59c \| somatic clone phenotype, suppressible \| partially \| somatic clone by Tm1^ZCL0722 (Stephan et al., 2012) psidin^55D4 has border follicle cell \| somatic clone phenotype, suppressible \| partially \| somatic clone by Tm1^ZCL0722 (Kim et al., 2011) psidin^55D4 has lamellipodium phenotype, suppressible by Tm1^ZCL0722 (Stephan et al., 2012)
Suppressor of
Statement Reference psidin^55D4 is a suppressor \| partially \| somatic clone of border follicle cell \| somatic clone phenotype of Tm1^ZCL0722 (Kim et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference The reduction in lamellipodia size and increase in neurite length which is seen in neurons in primary neuron cultures derived from psidin[55D4] embryos are suppressed if the embryos are also mutant for Tm1[ZCL0722]. psidin[55D4] Tm1[ZCL0722] double mutant Or59c olfactory receptor neuron (ORNs) show a marked reduction in the penetrance and severity of axonal targeting defects compared to psidin[55D4] single mutant ORNs, but the reduction in the number of Or59c ORNs seen in psidin[55D4] single mutant ORNs is not rescued in the double mutants. (Stephan et al., 2012) Border follicle cells simultaneously homozygous for both Tm1[ZCL0722] and psidin[55D4] show less severe migration defects than single mutant border follicle cells. (Kim et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	psidin^e02846 (Kim et al., 2011)
Rescued by	psidin^55D4 is rescued by psidin^Scer\UAS.cKa/Scer\GAL4^slbo.2.6 (Kim et al., 2011) psidin^85D1/psidin^55D4 is rescued by psidin^Scer\UAS.cKa/Scer\GAL4^Act.PU (Kim et al., 2011)
Comments	Expression of psidin[Scer\UAS.cKa] under the control of Scer\GAL4[slbo.2.6] rescues the migration defects seen in homozygous psidin[55D4] border cells. Expression of psidin[Scer\UAS.cKa] under the control of Scer\GAL4[Act.PU] rescues the lethality of psidin[55D4]/psidin[85D1] animals. (Kim et al., 2011)
Stocks ( 1 )
Bloomington	41123 w¹¹¹⁸; P{neoFRT}82B psidin^55D4 e^s/TM3, Sb¹ Ser¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	psid^55D4 (Kim et al., 2011) psidin^55D4 (Stephan et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Stephan et al., 2012, J. Neurosci. 32(46): 16080--16094 Drosophila Psidin Regulates Olfactory Neuron Number and Axon Targeting through Two Distinct Molecular Mechanisms. [FBrf0220017] Kim et al., 2011, Genes Dev. 25(7): 730--741 Psidin, a conserved protein that regulates protrusion dynamics and cell migration. [FBrf0213388]

Allele Dmel\psidin55D4

Allele Dmel\psidin^55D4