Allele Dmel\psidin^Scer\UAS.cKa

General Information
Symbol	Dmel\psidin^Scer\UAS.cKa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Kim	FlyBase ID	FBal0277251
Feature type	allele	Associated gene	Dmel\psidin

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kim et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the psidin AT25164 cDNA. (Kim et al., 2011)
Carried in construct	P{UAS-psid.K} (Kim et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	border follicle cell, with Scer\GAL4^slbo.2.6 (Kim et al., 2011)
Detailed Description
	Statement Reference Border follicle cells expressing psidin[Scer\UAS.cKa] under the control of Scer\GAL4[slbo.2.6] at 29[o]C have longer actin-rich protrusions than control cells. The protrusions are highly irregular in morphology, as they are elongated, irregular in length, of variable thickness and can even be branched. (Kim et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Statement Reference Act5C^{slbo.T:Avic\GFP-EGFP}, Scer\GAL4^slbo.2.6, psidin^Scer\UAS.cKa has border follicle cell phenotype (Kim et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Act5C[slbo.T:Avic\GFP-EGFP] and of psidin[Scer\UAS.cKa] under the control of Scer\GAL4[slbo.2.6] results in a strong inhibition of border follicle cell migration. Expression of psidin[Scer\UAS.cKa] under the control of Scer\GAL4[slbo.2.6] in a tsr[1], ssh[1-63], WASp[3], Hem[J4-48] or zip[1] heterozygous background has little or no effect on border cell migration. Expression of psidin[Scer\UAS.cKa] under the control of Scer\GAL4[slbo.2.6] in a Rac1[J11] Rac2[Δ] double heterozygous background has little or no effect on border cell migration. (Kim et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	psidin^Scer\UAS.cKa/Scer\GAL4^Act.PU rescues psidin^85D1/psidin^55D4 (Kim et al., 2011) psidin^Scer\UAS.cKa/Scer\GAL4^slbo.2.6 rescues psidin^55D4 (Kim et al., 2011)
Comments	Expression of psidin[Scer\UAS.cKa] under the control of Scer\GAL4[slbo.2.6] rescues the migration defects seen in homozygous psidin[55D4] border cells. Expression of psidin[Scer\UAS.cKa] under the control of Scer\GAL4[Act.PU] rescues the lethality of psidin[55D4]/psidin[85D1] animals. (Kim et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	psidin^Scer\UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Kim
Secondary FlyBase IDs

References ( 1 )
Research paper	Kim et al., 2011, Genes Dev. 25(7): 730--741 Psidin, a conserved protein that regulates protrusion dynamics and cell migration. [FBrf0213388]

Allele Dmel\psidinScer\UAS.cKa

Allele Dmel\psidin^Scer\UAS.cKa