Allele Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}

General Information
Symbol	Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}	Species	a. artificial
Name		FlyBase ID	FBal0277306
Feature type	allele	Associated gene	Zzzz\CAG

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (van Eyk et al., 2011) in vitro construct - regulatory fusion (van Eyk et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a Zzzz\CAG-repeat encoding 93 glutamines. The repeats are flanked by six amino acids on the N-terminal side and four on the C-terminal, and the T:Hsap\MYC and T:Zzzz\FLAG epitope tags are located downstream. (van Eyk et al., 2011)
Carried in construct	P{UAS-CAG.93} (van Eyk et al., 2011)
Tagged with	T:Hsap\MYC (van Eyk et al., 2011) T:Zzzz\FLAG (van Eyk et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	viable, with Scer\GAL4^GMR.PFa (van Eyk et al., 2011) visible, with Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
Detailed Description
	Statement Reference Expression of Zzzz\CAG[93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] results in pigmentation loss in the eye. (van Eyk et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, enhanceable by mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, enhanceable by sgg^Scer\UAS.cBa/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-enhanceable by Mef2^GD5039, Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-enhanceable by sgg^KK108994/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
Suppressed by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has viable phenotype, suppressible \| partially by mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-suppressible by Mef2^GD5039, Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible phenotype, non-suppressible by sgg^KK108994/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, enhanceable by mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, enhanceable by sgg^Scer\UAS.cBa/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-enhanceable by Mef2^GD5039, Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-enhanceable by sgg^KK108994/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-suppressible by Mef2^GD5039, Scer\GAL4^GMR.PFa (van Eyk et al., 2011) Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-suppressible by sgg^KK108994/Scer\GAL4^GMR.PFa (van Eyk et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Mef2[GD5039] with Zzzz\CAG[93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] does not alter the appearance of the eye. Co-expression of mod(mdg4)[GD16547] with Zzzz\CAG[93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] results in an enhancement of the Zzzz\CAG[93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG]-induced eye phenotype and a reduction in viability of the flies. Co-expression of sgg[Scer\UAS.cBa] with Zzzz\CAG[93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] results in an increase in the eye compared with ectopic expression of sgg[Scer\UAS.cBa] alone. There appears to be a reduction in the amount of pigment in the eye and in most cases there are necrotic patches and nearly complete loss of ommatidial array structure. Co-expression of sgg[KK108994] with Zzzz\CAG[93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] does not dramatically alter the exterior appearance of the eye compared with flies expressing Zzzz\CAG[93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] alone. (van Eyk et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	van Eyk et al., 2011, Hum. Mol. Genet. 20(14): 2783--2794 Perturbation of the Akt/Gsk3-{beta} signalling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs. [FBrf0213942]

Allele Zzzz\CAG93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG

Allele Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}