Allele Dmel\stmA^{S358A.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\stmA^{S358A.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0277668
Feature type	allele	Associated gene	Dmel\stmA

Allele class
Mutagen	in vitro construct - genomic fragment, in vitro construct - coding region fusion, in vitro construct - site directed mutagenesis
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Vijayakrishnan et al., 2010) in vitro construct - genomic fragment (Vijayakrishnan et al., 2010) in vitro construct - site directed mutagenesis (Vijayakrishnan et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: S358A. (Vijayakrishnan et al., 2010) Nucleotide substitution: A?G. (Vijayakrishnan et al., 2010) Nucleotide substitution: G?C. (Vijayakrishnan et al., 2010) A genomic fragment of stmA with the native promoter and a mutation within the lipase domain is tagged with a 3'-terminal T:Avic\GFP[EGFP] sequence and cloned into the pUAST plasmid. (Vijayakrishnan et al., 2010)
Carried in construct	P{stmA-EGFP.S358A} (Vijayakrishnan et al., 2010)
Tagged with	T:Avic\GFP^EGFP (Vijayakrishnan et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage (Vijayakrishnan et al., 2010) lethal \| first instar larval stage (Vijayakrishnan et al., 2010) viable, with stmA¹, stmA^rev499 (Vijayakrishnan et al., 2010) viable, with stmA¹/stmA^rev499 (Vijayakrishnan et al., 2010) viable (with stmA^rev499), with stmA¹ (Vijayakrishnan et al., 2010)
Phenotype Manifest In

Detailed Description
	Statement Reference stmA[rev499]/stmA[1] mutants carrying stmA[S358A.T:Avic\GFP-EGFP] are viable. At permissive temperature (25[o]C) stmA[1] flies expressing stmA[S358A.T:Avic\GFP-EGFP] in the Garland cells take TR-vidin tracer into discrete puncta/vesicles to the same level as wild-type controls. At the 37[o]C restrictive temperature, while wild-type cells continue to display robust tracer uptake, no tracer-positive vesicles are found. This indicates that there is a complete block in Garland cell endocytosis in stmA[1] flies carrying stmA[S358A.T:Avic\GFP-EGFP]. (Vijayakrishnan et al., 2010)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	stmA^{S358A.T:Avic\GFP-EGFP} rescues stmA¹/stmA^rev499 (Vijayakrishnan et al., 2010)
Fails to rescue	stmA^{S358A.T:Avic\GFP-EGFP} fails to rescue stmA¹ (Vijayakrishnan et al., 2010) stmA^{S358A.T:Avic\GFP-EGFP} fails to rescue stmA^rev499 (Vijayakrishnan et al., 2010)
Comments	stmA[S358A.T:Avic\GFP-EGFP]-expressing stmA[1]/stmA[rev499] animals behave as wild-type, with no paralysis, compared to stmA[1]/stmA[rev499] mutants, that experience paralysis within 5 minutes of exposure to the restrictive temperature. (Vijayakrishnan et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	stmA^{S358A.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Vijayakrishnan et al., 2010, Traffic 11(12): 1567--1578 Drosophila rolling blackout displays lipase domain-dependent and -independent endocytic functions downstream of dynamin. [FBrf0212267]

Allele Dmel\stmAS358A.T:Avic\GFP-EGFP

Allele Dmel\stmA^{S358A.T:Avic\GFP-EGFP}