Allele Dmel\E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0277725
Feature type	allele	Associated gene	Dmel\E2f

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Davidson and Duronio, 2012) in vitro construct - coding region fusion (Davidson and Duronio, 2012) in vitro construct - regulatory fusion (Davidson and Duronio, 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of a mutated form of E2f which contains the amino acid replacements I153A, Y156A and Y157A. Four amino acids in the RRXYD motif have also been replaced by alanine (R292A, R293A, Y295A, D296A). The protein is also tagged with T:Avic\GFP[EGFP]. (Davidson and Duronio, 2012)
Carried in construct	P{UASp-GFP-E2f.PIP-3A-DBD} (Davidson and Duronio, 2012)
Tagged with	T:Avic\GFP^EGFP (Davidson and Duronio, 2012)
Cytology
Phenotypic Data
Phenotypic Class
	cell death defective \| third instar larval stage, with Scer\GAL4^en-e16E (Davidson and Duronio, 2012)
Phenotype Manifest In
	wing disc \| posterior \| third instar larval stage, with Scer\GAL4^en-e16E (Davidson and Duronio, 2012)
Detailed Description
	Statement Reference The rate of proliferation in trypsin-dissociated posterior wing disc cells expressing E2f[PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[en-e16E] is similar to controls. A comparable proportion of cells have a G1 DNA content compared to controls. Expression of E2f[PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[en-e16E] induces apoptosis in the posterior compartment of intact third instar larval wing discs. A similar increase in apoptosis is seen when the wing disc cells are trypsin-dissociated and quantified with flow cytometry. The cells of the second mitotic wave in flies expressing E2f[PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[GMR.PU] show the stereotypical pattern of S phase seen in controls. As in wild type very few cells display DNA damage, however an increase in apoptosis is seen in the cells posterior to the morphogenetic furrow. (Davidson and Duronio, 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}, Scer\GAL4^en-e16E has cell death defective \| third instar larval stage phenotype, suppressible \| partially by Df(3L)H99/+ (Davidson and Duronio, 2012) E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}, Scer\GAL4^en-e16E has cell death defective \| third instar larval stage phenotype, suppressible \| partially by W⁰⁵⁰¹⁴/W[+] (Davidson and Duronio, 2012)
Other
Statement Reference BacA\p35^Scer\UAS.cHa, E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}, Scer\GAL4^en-e16E has viable phenotype (Davidson and Duronio, 2012)
Phenotype Manifest In
Suppressed by
Statement Reference E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}, Scer\GAL4^en-e16E has wing disc \| posterior \| third instar larval stage phenotype, suppressible \| partially by Df(3L)H99/+ (Davidson and Duronio, 2012) E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}, Scer\GAL4^en-e16E has wing disc \| posterior \| third instar larval stage phenotype, suppressible \| partially by W⁰⁵⁰¹⁴/W[+] (Davidson and Duronio, 2012)
Additional Comments
Genetic Interactions
Statement Reference One copy of W[05014] partially suppresses the apoptosis seen in the posterior compartment of third instar larval wing discs expressing E2f[PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[en-e16E]. One copy of Df(3L)H99 partially suppresses the apoptosis seen in the posterior compartment of third instar larval wing discs expressing E2f[PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[en-e16E]. (Davidson and Duronio, 2012)
Xenogenetic Interactions
Statement Reference Wing discs co-expressing E2f[PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP] and BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[en-e16E] appear largely normal. Wing discs co-expressing E2f[DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP] and BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[en-e16E] appear largely normal. (Davidson and Duronio, 2012)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Davidson and Duronio, 2012, PLoS Genet. 8(8): e1002831 S phase-coupled e2f1 destruction ensures homeostasis in proliferating tissues. [FBrf0219273]

Allele Dmel\E2fPIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP

Allele Dmel\E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}