Flies expressing either the P{UAS-Irk2.DN}5.1 or P{UAS-Irk2.DN}5.2 insertion lines of P{UAS-Irk2.DN} are lethal when crossed to Scer\GAL4Act5C.PI. The P{UAS-Irk2.DN}13 insertion line survives to adulthood when raised at 25[o]C, although only 20% survive when flies are raised at 18[o]C.
Only 17% of larvae expressing Irk2DN.Scer\UAS (insertion line P{UAS-Irk2.DN}5.1) under the control of Scer\GAL4Act5C.PI are able to hatch. No larvae survive beyond 48 hours.
All of the female flies resulting from the expression of P{UAS-Irk2.DN} (insertion line P{UAS-Irk2.DN}5.1 in the wing disc under the control of Scer\GAL4Bx-MS1096 at 25[o]C display wing defects. 6% of wings display moderate defects, which include wings that are small, collapsed or blistered, as well as those showing branching of the posterior cross-vein, L2-3 collapse, L4 bifurcation, incomplete L5 vein and wing bristle transformations. 94% of flies showed more severe defects, including partial or complete loss of the wing blade. For the P{UAS-Irk2.DN}5.2 insertion line, 42% show moderate wing defects and only 4% have severe defects. The phenotypes are more severe in males, and when flies are raised at lower temperatures.
Expression of P{UAS-Irk2.DN} (insertion line P{UAS-Irk2.DN}5.2) under the control of Scer\GAL4en-e16E causes wing vein and blistering defects.
Expression of Irk2DN.Scer\UAS (insertion line P{UAS-Irk2.DN}5.1) under the control of either Scer\GAL4en-e16E or Scer\GAL4A9 results in a reduction in wing disc size. Less than 5% of either genotype survives beyond the early larval stage. Expression of the P{UAS-Irk2.DN}13 under the control of Scer\GAL4A9 insertion line also results in a reduction in disc size.
Wing discs expressing Irk2DN.Scer\UAS (P{UAS-Irk2.DN}5.1) under the control of Scer\GAL4Bx-MS1096 are fragile and thinned in the wing pouch region. Apoptosis (TUNEL staining) occurs in the wing pouch area.
Irk2DN.UAS, Scer\GAL4Bx-MS1096 has visible | adult stage phenotype, enhanceable by dpp[+]/dpphr92
Irk2DN.UAS, Scer\GAL4Bx-MS1096 has visible | adult stage phenotype, enhanceable by tkv7/tkv[+]
Irk2DN.UAS, Scer\GAL4Bx-MS1096 has abnormal cell death | third instar larval stage phenotype, suppressible by BacA\p35UAS.cHa, Scer\GAL4Bx-MS1096
Irk2DN.UAS, Scer\GAL4Bx-MS1096 has visible | adult stage phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4Bx-MS1096
Irk2DN.UAS, Scer\GAL4ptc-559.1 is a suppressor of increased cell number | larval stage phenotype of RetMEN2B.UAS, Scer\GAL4ptc-559.1
Scer\GAL4ptc-559.1/Irk2DN.UAS is a suppressor of increased mortality during development phenotype of RetMEN2B.UAS
Scer\GAL4ptc-559.1/Irk2DN.UAS is a suppressor of partially lethal - majority die phenotype of RetMEN2B.UAS
Irk2DN.UAS, Scer\GAL4Bx-MS1096 has wing phenotype, enhanceable by dpp[+]/dpphr92
Irk2DN.UAS, Scer\GAL4Bx-MS1096 has wing phenotype, enhanceable by tkv7/tkv[+]
Irk2DN.UAS, Scer\GAL4Bx-MS1096 has wing disc | third instar larval stage phenotype, suppressible by BacA\p35UAS.cHa, Scer\GAL4Bx-MS1096
Irk2DN.UAS, Scer\GAL4Bx-MS1096 has wing | adult stage phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4Bx-MS1096
Irk2DN.UAS, Scer\GAL4ptc-559.1 is a suppressor of anterior-posterior compartment boundary of the wing disc phenotype of RetMEN2B.UAS, Scer\GAL4ptc-559.1
One copy of dpphr92 enhances the wing defects seen when Irk2DN.Scer\UAS is expressed under the control of Scer\GAL4Bx-MS1096. 84% of wings from female flies expressing the P{UAS-Irk2.DN}5.2 insertion line display defects, with 29% of these considered to be severe. When the stronger P{UAS-Irk2.DN}5.1 insertion line is expressed 97% of wings from female flies display severe defects.
One copy of tkv7 enhances the wing defects seen when Irk2DN.Scer\UAS is expressed under the control of Scer\GAL4Bx-MS1096. Total wing defects in females expressing the P{UAS-Irk2.DN}5.2 insertion line are increased from 46% to 72% of wings. Total wing defects in females expressing the stronger P{UAS-Irk2.DN}5.1 line are increased from 94% to 99% of wings.
Expression of BacA\p35Scer\UAS.cHa suppresses the apoptosis seen in wing discs expressing Irk2DN.Scer\UAS under the control of Scer\GAL4Bx-MS1096, although apoptosis is still seen in the periphery of the wing outside of the region of Scer\GAL4Bx-MS1096 expression. The severe wing phenotypes are not suppressed.
