FlyBase Allele Report: Dmel\parvin[UASp.mGFP6]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\parvin^UASp.mGFP6

General Information

Symbol

Dmel\parvin^UASp.mGFP6

Species

D. melanogaster

Name

FlyBase ID

FBal0277795

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{UASp-parvin.WT.mGFP6}

Also Known As

UAS::Parvin-GFP

Key Links

Transgenic product class

(Vakaloglou et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Vakaloglou et al., 2012)

Mutagen

in vitro construct

(Vakaloglou et al., 2012)

Progenitor genotype

Carried in construct

P{UASp-parvin.WT.mGFP6}

Cytology

Description

UASp regulatory sequences drive expression of parvin which is tagged at the C-terminal end with mGFP6.

(Vakaloglou et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Tagged with

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Chountala et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | larval stage, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

abnormal cell shape | larval stage, with Scer\GAL4^en.PU

(Chountala et al., 2012)

increased cell death | larval stage, with Scer\GAL4^en.PU

(Chountala et al., 2012)

increased cell death | larval stage, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

lethal - all die before end of larval stage, with Scer\GAL4^drm.7.1

(Chountala et al., 2012)

lethal - all die before end of larval stage, with Scer\GAL4^how-24B

(Chountala et al., 2012)

lethal - all die before end of larval stage, with Scer\GAL4^Mef2.PU

(Chountala et al., 2012)

partially lethal - majority die, with Scer\GAL4^en.PU

(Chountala et al., 2012)

some die during pupal stage, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

visible | adult stage, with Scer\GAL4^69B

(Chountala et al., 2012)

visible | adult stage, with Scer\GAL4^en.PU

(Chountala et al., 2012)

visible | adult stage, with Scer\GAL4^GMR.long

(Chountala et al., 2012)

visible | adult stage, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

Phenotype Manifest In

anterior-posterior compartment boundary of the wing disc, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

arista, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

eye, with Scer\GAL4^69B

(Chountala et al., 2012)

eye, with Scer\GAL4^GMR.long

(Chountala et al., 2012)

eye, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

filamentous actin, with Scer\GAL4^en.PU

(Chountala et al., 2012)

filamentous actin, with Scer\GAL4^GMR.long

(Chountala et al., 2012)

leg, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

maxillary palp, with Scer\GAL4^48Y

(Chountala et al., 2012)

ocellar bristle, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

ommatidium, with Scer\GAL4^GMR.long

(Chountala et al., 2012)

posterior crossvein, with Scer\GAL4^69B

(Chountala et al., 2012)

scutellar bristle, with Scer\GAL4^ptc.PU

(Chountala et al., 2012)

wing, with Scer\GAL4^en.PU

(Chountala et al., 2012)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Chountala et al., 2012)

wing vein, with Scer\GAL4^en.PU

(Chountala et al., 2012)

Detailed Description

Statement

Reference

The expression of parvin^UASp.mGFP6 under the control of Scer\GAL4^en.PU leads to wing defects in the surviving adults, including notches and vein defects, as compared to controls; these are associated with increased apoptosis, epithelial disorganization and basal F-actin disorganization in the larval wing disc posterior compartment, as compared to the neighboring anterior compartment or as compared to controls.

The expression of parvin^UASp.mGFP6 under the control of Scer\GAL4^ptc.PU induces a mild rough eye phenotype, loss of thoracic bristles (namely in the scutellum) and ocellar bristle, loss of the arista, and leg dysplasia, as compared to controls; in the larval wing disc, the expressing cells (normally located along the anterior-posterior boundary) extrude towards the basal side of the epithelium and invade proximal areas, as compared to controls.

The expression of parvin^UASp.mGFP6 also leads to: the segregation of cuticular vesicles and the loss of the maxillary palpus (Scer\GAL4^48Y-driven expression); a mild rough eye and the absence of the posterior crossvein (Scer\GAL4^69B-driven expression); and a rough eye phenotype, associated with distortion of ommatidia and disorganization of basal F-actin in pigment cells (Scer\GAL4^GMR.long-driven expression). However, expression under the control of either Scer\GAL4^elav.PU or Scer\GAL4^sev.PU does not lead to any obvious phenotype compared to controls.

(Chountala et al., 2012)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has partially lethal - majority die phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has increased cell death | larval stage phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has partially lethal - majority die phenotype, suppressible | partially by Ilk^UAS.cZa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has increased cell death | larval stage phenotype, suppressible | partially by Ilk^UAS.cZa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has partially lethal - majority die phenotype, suppressible by Ilk^UAS.cZa/Diap1^UAS.cUa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has increased cell death | larval stage phenotype, suppressible by Ilk^UAS.cZa/Diap1^UAS.cUa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, enhanceable by mew^UAS.cMBa/Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, enhanceable by Df(3R)e-GC3/+

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, enhanceable by Eip93F^GD4449, Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, enhanceable by Scer\GAL4^GMR.long/mys^KK100518

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, enhanceable by Zasp52^KK101276/Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, enhanceable by Zasp52^GD14816/Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, enhanceable by Chd64^GD1212, Scer\GAL4^GMR.long

(Chountala et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, suppressible | partially by Hsap\PTEN^C124S.UAS, Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has wing disc posterior compartment phenotype, suppressible by Ilk^UAS.cZa/Diap1^UAS.cUa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has filamentous actin phenotype, suppressible | partially by Ilk^UAS.cZa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has filamentous actin phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, suppressible by wech^UAS.EGFP/Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, suppressible | partially by Ilk^UAS.cZa, Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, suppressible by Pten^UAS.cUa, Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, suppressible by Df(3L)Exel6119/+

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, suppressible by Df(3R)ED2/+

(Chountala et al., 2012)

Scer\GAL4^GMR.long, parvin^UASp.mGFP6 has eye phenotype, suppressible by Xrp1^KK104477/Scer\GAL4^GMR.long

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has wing disc posterior compartment phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^en.PU

(Chountala et al., 2012)

Scer\GAL4^en.PU, parvin^UASp.mGFP6 has wing disc posterior compartment phenotype, suppressible | partially by Ilk^UAS.cZa, Scer\GAL4^en.PU

(Chountala et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^69B, parvin^UASp.mGFP6 has posterior crossvein phenotype, non-suppressible by Scer\GAL4^en.PU/Ilk^UAS.cZa/Diap1^UAS.cUa

(Chountala et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^how-24B/parvin^UASp.mGFP6 rescues parvin^unspecified

(Vakaloglou et al., 2012)

Comments

Expression of parvin^{Scer\UAS.P\T.T:Avic\GFP-m6} under the control of Scer\GAL4^how-24B parvin^unspecified animals to adulthood.

(Vakaloglou et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

parvin^{Scer\UAS.P\T.T:Avic\GFP-m6}

parvin^UASp.mGFP6

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections