Allele Dmel\Nep4^Scer\UAS.A

General Information
Symbol	Dmel\Nep4^Scer\UAS.A	Species	D. melanogaster
Name		FlyBase ID	FBal0277820
Feature type	allele	Associated gene	Dmel\Nep4

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Panz et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the full-length Nep4 A isoform. (Panz et al., 2012)
Carried in construct	P{UAS-Nep4.A} (Panz et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage, with Scer\GAL4^Mef2.5xCD (Panz et al., 2012) locomotor behavior defective \| second instar larval stage, with Scer\GAL4^Mef2.5xCD (Panz et al., 2012)
Phenotype Manifest In
	embryonic/larval somatic muscle \| third instar larval stage, with Scer\GAL4^how-24B (Panz et al., 2012) embryonic/larval somatic muscle \| third instar larval stage, with Scer\GAL4^Mef2.5xCD (Panz et al., 2012) I band \| third instar larval stage, with Scer\GAL4^Mef2.5xCD (Panz et al., 2012) sarcomere \| third instar larval stage, with Scer\GAL4^Mef2.5xCD (Panz et al., 2012) sarcoplasmic reticulum \| third instar larval stage, with Scer\GAL4^Mef2.5xCD (Panz et al., 2012) Z disc \| third instar larval stage, with Scer\GAL4^Mef2.5xCD (Panz et al., 2012)
Detailed Description
	Statement Reference Expression of high levels of Nep4[Scer\UAS.A] under the control of Scer\GAL4[Mef2.5xCD] results in a reduced crawling speed in second instar larvae compared to wild type. This reduction appears to be caused by a combination of fewer and weaker muscle contractions than normal. The body wall muscles of third instar larvae expressing Nep4[Scer\UAS.A] under the control of Scer\GAL4[Mef2.5xCD] show degeneration and a loss of sarcomeric structure. Actin-myosin distribution is disorganised, the I-band pattern is completely disrupted and Z-bands are staggered. Nuclei with dispersed chromatin, swollen and severely fragmented mitochondria and swollen sarcoplasmic reticuli are seen. Larvae expressing Nep4[Scer\UAS.A] under the control of Scer\GAL4[how-24B] show muscle degeneration. (Panz et al., 2012)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Nep4^Scer\UAS.A
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Panz et al., 2012, Biol. Cell 104(9): 553--568 A novel role for the non-catalytic intracellular domain of Neprilysins in muscle physiology. [FBrf0219388]

Allele Dmel\Nep4Scer\UAS.A

Allele Dmel\Nep4^Scer\UAS.A