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General Information
Symbol
Dmel\diaΔDad.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0277860
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of dia lacking the C-terminal dia autoinhibitory domain. The protein is tagged at the N-terminal end with EGFP (the tag is joined to the dia coding region via a HRYTSLYKKAGSAAAPFT linker). Encodes a constitutively active protein.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4sns.PK results in defects in myoblast fusion. Fusion competent myoblasts show a decrease in the volume of F-actin foci and an increase in the frequency of filopodia emanating from an F-actin focus compared to controls.

Expression of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E results in a dramatic increase in the number of filopodia in the epidermal cells. The filopodia are no longer confined to leading edge cells but cover the apical surface of all epidermal cells. In the leading edge cells, filopodia are seen all over the cell, rather than being confined to the leading edge. At the leading edge, the number of filopodia is substantially increased and broad lamellipodia terminating in many filopodia are also seen. Individual filopodia are blunter than in wild type and the lifetime of each filopodium is more than doubled.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of enaScer\UAS.T:Avic\GFP and diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos induces protrusions in epidermal cells that are different from those produced by expression of either construct alone. Co-expression results in lamellipodia both at the leading edge and in more ventral epidermal cells, at the expense of filopodia.

Xenogenetic Interactions
Statement
Reference

Co-expression of Zzzz\actAFP4mito.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E does not suppress the ability of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP to induce elevated numbers of filopodia on leading edge cells or on more ventral epidermal cells. The lifetime of the filopodia is still longer than normal in the co-expressing embryos. The number of lamellipodia and lamellipodial area is significantly reduced in the co-expressing leading edge cells compared to wild type.

Complementation and Rescue Data
Comments

Expression of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP in the sensory organ precursor lineage (using Scer\GAL4neur-GAL4-A101) in large homozygous dia5 clones in the leg restores socket cell protrusions and the formation of associated bract cells in the clones.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP
diaΔDad.UASp.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (6)