Co-expression of enaScer\UAS.T:Avic\GFP and diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos induces protrusions in epidermal cells that are different from those produced by expression of either construct alone. Co-expression results in lamellipodia both at the leading edge and in more ventral epidermal cells, at the expense of filopodia.
Co-expression of Zzzz\actAFP4mito.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E does not suppress the ability of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP to induce elevated numbers of filopodia on leading edge cells or on more ventral epidermal cells. The lifetime of the filopodia is still longer than normal in the co-expressing embryos. The number of lamellipodia and lamellipodial area is significantly reduced in the co-expressing leading edge cells compared to wild type.