Allele Dmel\dia^{FH3FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\dia^{FH3FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0277861
Feature type	allele	Associated gene	Dmel\dia

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Homem and Peifer, 2009) in vitro construct - deletion (Homem and Peifer, 2009) in vitro construct - regulatory fusion (Homem and Peifer, 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of dia lacking the C-terminal dia autoinhibitory domain and the N-terminal GTPase-binding domain. The protein is tagged at the N-terminal end with T:Avic\GFP[EGFP] (the tag is joined to the dia coding region via a HRYTSLYKKAGSAAAPFT linker). (Homem and Peifer, 2009)
Carried in construct	P{UASp-dia.FH3FH1FH2.EGFP} (Homem and Peifer, 2009)
Tagged with	T:Avic\GFP^EGFP (Homem and Peifer, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^e22c (Homem and Peifer, 2009)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of dia[FH3FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[en-e16E] does not induce significant numbers of ectopic filopodia at the leading edge and there is little effect on protrusions of epidermal cells ventral to the leading edge. Lamellipodial area and filopodial number is similar to wild type in leading edge cells. Filopodial lifetime is significantly increased compared to wild type. (Homem and Peifer, 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	dia^{FH3FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Homem and Peifer, 2009, Mol. Biol. Cell 20(24): 5138--5155 Exploring the Roles of Diaphanous and Enabled Activity in Shaping the Balance between Filopodia and Lamellipodia. [FBrf0209542]

Allele Dmel\diaFH3FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP

Allele Dmel\dia^{FH3FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}