Allele Dmel\dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0277863
Feature type	allele	Associated gene	Dmel\dia

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Homem and Peifer, 2009) in vitro construct - deletion (Homem and Peifer, 2009) in vitro construct - regulatory fusion (Homem and Peifer, 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of the FH1 and FH2 domains of dia. The protein is tagged at the N-terminal end with T:Avic\GFP[EGFP] (the tag is joined to the dia coding region via a HRYTSLYKKAGSAAAPFT linker). (Homem and Peifer, 2009)
Carried in construct	P{UASp-dia.FH1FH2.EGFP} (Homem and Peifer, 2009)
Tagged with	T:Avic\GFP^EGFP (Homem and Peifer, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^e22c (Homem and Peifer, 2009)
Phenotype Manifest In
	embryonic leading edge cell, with Scer\GAL4^en-e16E (Homem and Peifer, 2009) epidermal cell, with Scer\GAL4^en-e16E (Homem and Peifer, 2009) filopodium, with Scer\GAL4^en-e16E (Homem and Peifer, 2009) lamellipodium, with Scer\GAL4^en-e16E (Homem and Peifer, 2009)
Detailed Description
	Statement Reference Expression of dia[FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[en-e16E] results in the dynamic lamellipodia and filopodia of the leading edge cells being replaced by lamellipodia with ruffled edges and by fan-shaped lamellipodia with radial actin arrays not seen in wild-type lamellipodia. These lamellipodia are larger than in wild type. Filopodial number, length and lifetime are all significantly decreased compared to wild type. Many of the remaining "filopodia" arise from fan-shaped lamellipodia and do not grow substantially after their appearance, in contrast to wild-type filopodia. (Homem and Peifer, 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^en-e16E, dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP} has embryonic leading edge cell phenotype, suppressible by Scer\GAL4^en-e16E/Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP} (Homem and Peifer, 2009) Scer\GAL4^en-e16E, dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP} has filopodium phenotype, suppressible by Scer\GAL4^en-e16E/Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP} (Homem and Peifer, 2009) Scer\GAL4^en-e16E, dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP} has lamellipodium phenotype, suppressible by Scer\GAL4^en-e16E/Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP} (Homem and Peifer, 2009)
Other
Statement Reference Scer\GAL4^en-e16E, dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}, ena^{Scer\UAS.T:Avic\GFP} has embryonic leading edge cell phenotype (Homem and Peifer, 2009) Scer\GAL4^en-e16E, dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}, ena^{Scer\UAS.T:Avic\GFP} has filopodium phenotype (Homem and Peifer, 2009) Scer\GAL4^en-e16E, dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}, ena^{Scer\UAS.T:Avic\GFP} has lamellipodium phenotype (Homem and Peifer, 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of ena[Scer\UAS.T:Avic\GFP] and dia[FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[en-e16E] in embryos results in fewer filopodia but larger lamellipodia at the leading edge. (Homem and Peifer, 2009)
Xenogenetic Interactions
Statement Reference Co-expression of Zzzz\actA[FP4mito.Scer\UAS.T:Avic\GFP-EGFP] largely restores filopodia number and length to wild type in leading edge cells expressing dia[FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[en-e16E]. Fewer ruffle-like lamellipodia are seen and those that form are nearer wild type in size. (Homem and Peifer, 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Homem and Peifer, 2009, Mol. Biol. Cell 20(24): 5138--5155 Exploring the Roles of Diaphanous and Enabled Activity in Shaping the Balance between Filopodia and Lamellipodia. [FBrf0209542]

Allele Dmel\diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP

Allele Dmel\dia^{FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP}