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General Information
Symbol
Dmel\diaFH1FH2.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0277863
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of the FH1 and FH2 domains of dia. The protein is tagged at the N-terminal end with EGFP (the tag is joined to the dia coding region via a HRYTSLYKKAGSAAAPFT linker).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E results in the dynamic lamellipodia and filopodia of the leading edge cells being replaced by lamellipodia with ruffled edges and by fan-shaped lamellipodia with radial actin arrays not seen in wild-type lamellipodia. These lamellipodia are larger than in wild type. Filopodial number, length and lifetime are all significantly decreased compared to wild type. Many of the remaining "filopodia" arise from fan-shaped lamellipodia and do not grow substantially after their appearance, in contrast to wild-type filopodia.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of enaScer\UAS.T:Avic\GFP and diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos results in fewer filopodia but larger lamellipodia at the leading edge.

Xenogenetic Interactions
Statement
Reference

Co-expression of Zzzz\actAFP4mito.Scer\UAS.T:Avic\GFP-EGFP largely restores filopodia number and length to wild type in leading edge cells expressing diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E. Fewer ruffle-like lamellipodia are seen and those that form are nearer wild type in size.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP
diaFH1FH2.UASp.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (2)