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General Information
Symbol
Dmel\mir-285UAS.DsRed(Unk)
Species
D. melanogaster
Name
FlyBase ID
FBal0278034
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing the mir-285 pre-miRNA hairpin has been inserted into the DsRed(Unk) 3'UTR.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression mir-285UAS.DsRed(Unk) under the control of Scer\GAL4ey.PU induces a small eye phenotype. Expression under the control of Scer\GAL4GMR.PU induces a rough eye phenotype associated with substantial apoptosis in the eye disc. Expression under the control of either Scer\GAL4hh.PU or Scer\GAL4en.PU induces wrinkled wings, associated with substantial apoptosis in the wing disc.

Expression under the control of Scer\GAL4moody.PU induces a compromised blood-brain barrier, as dye injected into the body cavity is able to diffuse into the adult retina or third instar larval brain. In agreement, larval subperineurial glial cells show disrupted/discontinuous septate junctions (Dlg immunostaining); these cells also show significant decreases in nuclear size and in ploidy only at higher expression conditions (i.e. at 25[o]C but not at 18[o]C), as compared to controls. There are also a significant increase in larval brain volume only at higher expression conditions (i.e. at 25[o]C but not at 18[o]C), as compared to controls.

Expression under the control of Scer\GAL4repo.PU results in a compromised larval blood-brain barrier, as injected dye into the body cavity is able to diffuse into the larval brain; no significant apoptosis is observed in the larval brain.

Adults expressing mir-285Scer\UAS.T:Disc\RFP under the control of Scer\GAL4Bx-MS1096 have curved wings.

Expression of mir-285Scer\UAS.T:Disc\RFP under the control of Scer\GAL4sd-SG29.1 results in curved and folded wings which are often broken.

Expression of mir-285Scer\UAS.T:Disc\RFP under the control of Scer\GAL4ptc-559.1 does not result in a mutant phenotype.

Expression of mir-285Scer\UAS.T:Disc\RFP under the control of Scer\GAL4Bx-MS1096 results in wing blisters.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
mir-285Scer\UAS.T:Disc\RFP
mir-285UAS.DsRed(Unk)
Name Synonyms
Secondary FlyBase IDs
    References (3)