UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing the mir-303 pre-miRNA hairpin has been inserted into the DsRed(Unk) 3'UTR.
lethal, with Scer\GAL4Bx-MS1096
lethal, with Scer\GAL4elav.PU
lethal, with Scer\GAL4ey.PU
lethal - all die before end of larval stage, with Scer\GAL4da.G32
lethal - all die during larval stage, with Scer\GAL4da.PU
some die during larval stage, with Scer\GAL4da.G32
visible, with Scer\GAL4ptc-559.1
visible, with Scer\GAL4sd-SG29.1
eye, with Scer\GAL4ey.PU
eye, with Scer\GAL4GMR.PU
haltere, with Scer\GAL4ptc.PU
macrochaeta, with Scer\GAL4sca.PU
macrochaeta | supernumerary, with Scer\GAL4Bx-MS1096
microchaeta, with Scer\GAL4EQ1
wing, with Scer\GAL4Bx-MS1096
wing, with Scer\GAL4ptc-559.1
wing, with Scer\GAL4ptc.PU
wing, with Scer\GAL4sd-SG29.1
wing margin, with Scer\GAL4sd.PU
wing margin, with Scer\GAL4sd-SG29.1
wing vein, with Scer\GAL4Bx-MS1096
Expression via Scer\GAL4Bx-MS1096 results in lethality/extra macrochaetes in notum and small, curled, folded wings.
Expression via Scer\GAL4GMR.PU results in rough eyes
Expression via Scer\GAL4ptc.PU results in curled wings and big halteres.
Expression via Scer\GAL4EQ1 results in misorganized microchaetes.
Expression via Scer\GAL4sca.PU results in short/thin macrochaetes.
Expression via Scer\GAL4ey.PU results in lethality and a mild eye phenotype.
Expression via Scer\GAL4sd.PU results in an irregular wing margin.
Expression of mir-303Scer\UAS.T:Disc\RFP under the control of Scer\GAL4Bx-MS1096 results in male lethality. Surviving females have wings which are usually very small, folded and have strong defects in patterning.
Expression of mir-303Scer\UAS.T:Disc\RFP under the control of Scer\GAL4sd-SG29.1 results in small folded wings which may have an irregular wing margin.
Expression of mir-303Scer\UAS.T:Disc\RFP under the control of Scer\GAL4ptc-559.1 results in a curved wing.