FlyBase Allele Report: Dmel\shot[GL01286]

Expressing shot^GL01286 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} results in third instar larval adipocytes presenting extracellular matrix accumulations extending to the cell body (Vkg.GFP).

Expressing shot^GL01286 under the control of Scer\GAL4^Cg.PA results in third instar larval adipocytes presenting enlarged, deformed and highly eccentric nuclei, aberrant matrix accumulations (Trol.GFP) extending to the cell body, and plasma membrane invaginations that concentrates actin filaments, myosin and Rho1. In agreement, mitochondria, endoplasmic reticulum exit sites and endocytic or recycling vesicles lose their homogeneous distribution throughout the cytoplasm and seem to gravitate toward a single focus near the nucleus. These adipocytes also exhibit a large juxtanuclear focus of microtubules, including non-centrosomal minus-ends (Patronin.GFP), in contrast to a normal perinuclear distribution in controls.

Expressing shot^GL01286 under the control of Scer\GAL4^NP0001 and Scer\GAL4^Ugt36A1.PK causes aberrant juxtanuclear microtubule foci and mispositioned nuclei in the midgut enteroblasts and garland filter cells, respectively.

(Sun et al., 2019)

Expression of shot^GL01286 under the control of Scer\GAL4^elav.PU results in overgrowth (increase in bouton number) at the neuromuscular junction (NMJ) in third instar larvae, while expression under the control of Scer\GAL4^how-24B has no effect on bouton number at the NMJ.

Axons expressing shot^GL01286 under the control of Scer\GAL4^tey-5053A show enhanced regenerative axonal sprouting compared to controls after a crushing injury.

(Valakh et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, shot^GL01286 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Patronin^HMS01547, Scer\GAL4^RapGAP1-OK6

(Gao et al., 2024)

NOT Suppressor of

Statement

Reference

shot^GL01286, Scer\GAL4^RapGAP1-OK6 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Patronin^HMS01547, Scer\GAL4^RapGAP1-OK6

(Gao et al., 2024)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, shot^GL01286 has NMJ bouton | larval stage phenotype, enhanceable by Patronin^HMS01547, Scer\GAL4^RapGAP1-OK6

(Gao et al., 2024)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, enhanceable by Rab11^JF02812, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, enhanceable by Rab11^JF02812, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, enhanceable by Rab11^JF02812, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Suppressed by

Statement

Reference

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by shi^NIG.18102R/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, suppressible by shi^NIG.18102R/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by shi^NIG.18102R/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Rab5^HMS00147, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, suppressible by Rab5^HMS00147, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by Rab5^HMS00147, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PA/sqh^HMS00437

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PA/sqh^HMS00437

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PA/sqh^HMS00437

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Rho1^HMS00375, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, suppressible by Rho1^HMS00375, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by Rho1^HMS00375, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Septin2^JF02838/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, suppressible by Septin2^JF02838/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by Septin2^JF02838/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PA/pnut^HMC05924

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PA/pnut^HMC05924

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PA/pnut^HMC05924

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by dia^HM05027/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, suppressible by dia^HM05027/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by dia^HM05027/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by βTub85D^NIG.9359R, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by βTub85D^NIG.9359R, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Patronin^HMS01547, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by Patronin^HMS01547, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has microtubule | third instar larval stage phenotype, suppressible | partially by Patronin^HMS01547, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Cg.PA/kat-60L1^HMS00510

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has microtubule | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Cg.PA/kat-60L1^HMS00510

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Cg.PA/kat-60L1^{UAS.long.mRFP(Unk)}

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has microtubule | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Cg.PA/kat-60L1^{UAS.long.mRFP(Unk)}

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has microtubule | third instar larval stage phenotype, suppressible by Msp300^HMS00368, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PA/klar^GD9271

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has microtubule | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PA/klar^GD9271

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by koi^GD1835/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has microtubule | third instar larval stage phenotype, suppressible by koi^GD1835/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^{SPARC-MI00329-GAL4}, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Khc^KO.mutA, Scer\GAL4^{SPARC-MI00329-GAL4}

(Sun et al., 2019)

Scer\GAL4^{SPARC-MI00329-GAL4}, shot^GL01286 has microtubule | third instar larval stage phenotype, suppressible by Khc^KO.mutA, Scer\GAL4^{SPARC-MI00329-GAL4}

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, suppressible by Act87E^HMS02488, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, suppressible by Act87E^HMS02488, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has nucleus | third instar larval stage phenotype, suppressible by Act87E^HMS02488, Scer\GAL4^Cg.PA

(Sun et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, non-suppressible by scra^GL01269/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has endoplasmic reticulum | third instar larval stage phenotype, non-suppressible by scra^GL01269/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, non-suppressible by kat-60L1^{UAS.short.mRFP(Unk)}/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has microtubule | third instar larval stage phenotype, non-suppressible by kat-60L1^{UAS.short.mRFP(Unk)}/Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^GL01286 has fat cell | third instar larval stage phenotype, non-suppressible by Msp300^HMS00368, Scer\GAL4^Cg.PA

(Sun et al., 2019)

NOT Suppressor of

Statement

Reference

shot^GL01286, Scer\GAL4^RapGAP1-OK6 is a non-suppressor of NMJ bouton | larval stage phenotype of Patronin^HMS01547, Scer\GAL4^RapGAP1-OK6

(Gao et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

The enhanced regenerative axonal sprouting seen in axons expressing shot^GL01286 under the control of Scer\GAL4^tey-5053A compared to controls after a crushing injury is eliminated if they are co-expressing wnd^VDRC.cUa.

(Valakh et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

shot^GL01286 is rescued by shot^{LA-Δrod1.UAS.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Lu et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

41858

y¹ v¹; P{TRiP.GL01286}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

shot^GL01286

(Zheng et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (12)

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