FlyBase Allele Report: Dmel\cyst[HMS01750]

General Information

Symbol

Dmel\cyst^HMS01750

Species

D. melanogaster

Name

FlyBase ID

FBal0278868

Feature type

allele

Associated gene

Dmel\cyst

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01750}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01750}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01750

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 21

modeled by Hsap\MATR3^{S85C.UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

modeled by Hsap\MATR3^{UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

modeled by Hsap\MATR3^{F115C.UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | embryonic stage | maternal effect, with Df(2L)BSC301, Scer\GAL4^{VP16.mat.αTub67C}

(Silver et al., 2019)

lethal - all die during embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Silver et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Maternal expression of cyst^HMS01750 under the control of Scer\GAL4^{VP16.mat.αTub67C} leads to embryonic lethality, associated with epithelial/cuticular defects, namely many small epithelial vesicles or cysts and small cuticle shields, instead of the large continuous epithelial sheet observed in controls. This cuticle phenotype is enhanced by maternal heterozygosity for Df(2L)BSC301.

The embryos show progressive adherens junction fragmentation during gastrulation ( DEca::GFP ) so that many cells, particularly in the ventral ectoderm, lose the Adherens Junctions; other cells show focal concentration of Adherens Junction components, suggesting the beginning of cyst formation. The epithelial cysts are first seen at the end of gastrulation and can be followed throughout the rest of development; these cysts show an apparently normal polarized distribution of Crb, aPKc, Arm and Yrt proteins.

While the maternal expression of cyst^HMS01750 under the control of Scer\GAL4^{VP16.mat.αTub67C} does not greatly affect the planar polarization of Baz protein in embryos, additional maternal heterozygosity for Df(2L)BSC301 leads to Baz hyper-polarization.

(Silver et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(2L)BSC301, Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750 has abnormal cell polarity | embryonic stage | maternal effect phenotype, enhanceable by aPKC^k06403/aPKC[+]

(Silver et al., 2019)

Suppressor of

Statement

Reference

cyst^HMS01750/Scer\GAL4^repo.PU is a suppressor | partially of lethal - all die during P-stage phenotype of Scer\GAL4^repo.PU, brm^GD4507

(Tegeder et al., 2019)

Other

Statement

Reference

Scer\GAL4^repo.PU, brm^GD4507, cyst^HMS01750 has partially lethal - majority die phenotype

(Tegeder et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(2L)BSC301, Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750 has embryonic epidermis | maternal effect phenotype, enhanceable by aPKC^k06403/aPKC[+]

(Silver et al., 2019)

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750 has embryonic epidermis | maternal effect | progressive phenotype, suppressible | partially by Scer\GAL4^{VP16.mat.αTub67C}/scrib^HMS01490

(Silver et al., 2019)

Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750 has embryonic/larval cuticle | maternal effect | embryonic stage phenotype, suppressible | partially by Scer\GAL4^{VP16.mat.αTub67C}/scrib^HMS01490

(Silver et al., 2019)

Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750 has embryonic epidermis | maternal effect | progressive phenotype, suppressible | partially by l(2)gl⁴/l(2)gl⁴

(Silver et al., 2019)

Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750 has embryonic/larval cuticle | maternal effect | embryonic stage phenotype, suppressible | partially by l(2)gl⁴/l(2)gl⁴

(Silver et al., 2019)

NOT Suppressor of

Statement

Reference

cyst^HMS01750/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of embryonic head | maternal effect phenotype of l(2)gl⁴

(Silver et al., 2019)

cyst^HMS01750/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of embryonic epidermis | maternal effect phenotype of Scer\GAL4^{VP16.mat.αTub67C}, scrib^HMS01490

(Silver et al., 2019)

cyst^HMS01750/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of embryonic/larval cuticle | maternal effect | embryonic stage phenotype of Scer\GAL4^{VP16.mat.αTub67C}, scrib^HMS01490

(Silver et al., 2019)

Other

Statement

Reference

Df(2L)BSC301, Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750 has embryonic/larval cuticle | maternal effect | embryonic stage phenotype

(Silver et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Maternal co-expression of cyst^HMS01750 and scrib^HMS01490 under the control of Scer\GAL4^{VP16.mat.αTub67C} leads to embryonic cuticle defects that are similar to those induced by the expression of scrib^HMS01490 alone (i.e. epidermal cell clusters surrounded by cuticle), and milder than those induced by the expression of cyst^HMS01750 alone.

(Silver et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

cyst^HMS01750 is rescued by cyst^{R.ΔPBM.N-sfGFP}

(Silver et al., 2019)

cyst^HMS01750 is rescued by cyst^R.C-sfGFP

(Silver et al., 2019)

cyst^HMS01750 is rescued by cyst^R.N-sfGFP

(Silver et al., 2019)