FlyBase Allele Report: Dmel\dlg1[HMS01954]

General Information

Symbol

Dmel\dlg1^HMS01954

Species

D. melanogaster

Name

FlyBase ID

FBal0279068

Feature type

allele

Associated gene

Dmel\dlg1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01954}

Also Known As

UAS-dlg-RNAi, UAS-Dlg RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01954}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01954

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Bunker et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is ameliorated by vg^UAS.cKa

(Bunker et al., 2015)

is NOT ameliorated by Cat^UAS.cAa

(Bunker et al., 2015)

is ameliorated by bsk^K53R.UAS

(Bunker et al., 2015)

is ameliorated by kay^pan-Ala.UAS

(Bunker et al., 2015)

is ameliorated by brm^{K804R.UAS.Tag:HA}

(Bunker et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

increased size | larval stage, with Scer\GAL4^hh-Gal4

(Sharp et al., 2021)

neoplasia | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

Phenotype Manifest In

wing disc | larval stage, with Scer\GAL4^hh-Gal4

(Sharp et al., 2021)

wing disc | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

Detailed Description

Statement

Reference

The expression of dlg1^HMS01954 driven by Scer\GAL4^Bx-MS1096 leads to the formation of tumors in the third instar larval wing disc, with overgrowth, tissue disorganization and expansion of the apical cortical domain, as compared to controls.

(Bunker et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hh-Gal4, dlg1^HMS01954 has increased size | larval stage phenotype, suppressible by E(bx)^HMS00065, Scer\GAL4^hh-Gal4

(Sharp et al., 2021)

Scer\GAL4^hh-Gal4, dlg1^HMS01954 has increased size | larval stage phenotype, suppressible by Iswi^HMS00628, Scer\GAL4^hh-Gal4

(Sharp et al., 2021)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has neoplasia | third instar larval stage phenotype, suppressible by Scer\GAL4^Bx-MS1096/vg^UAS.cKa

(Bunker et al., 2015)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has neoplasia | third instar larval stage phenotype, suppressible | partially by brm^{K804R.UAS.Tag:HA}/Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has neoplasia | third instar larval stage phenotype, non-suppressible by Cat^UAS.cAa, Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has neoplasia | third instar larval stage phenotype, non-suppressible by ey^UAS.cHa/Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has neoplasia | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/bsk^K53R.UAS

(Bunker et al., 2015)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has neoplasia | third instar larval stage phenotype, non-suppressible by kay^pan-Ala.UAS/Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hh-Gal4, dlg1^HMS01954 has wing disc | larval stage phenotype, suppressible | partially by E(bx)^HMS00065, Scer\GAL4^hh-Gal4

(Sharp et al., 2021)

Scer\GAL4^hh-Gal4, dlg1^HMS01954 has wing disc | larval stage phenotype, suppressible | partially by Iswi^HMS00628, Scer\GAL4^hh-Gal4

(Sharp et al., 2021)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4^Bx-MS1096/vg^UAS.cKa

(Bunker et al., 2015)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has wing disc | third instar larval stage phenotype, suppressible | partially by brm^{K804R.UAS.Tag:HA}/Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

NOT suppressed by

Statement

Reference

dlg1^HMS01954 has wing disc | third instar larval stage phenotype, non-suppressible by Cat^UAS.cAa/Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

dlg1^HMS01954 has wing disc | third instar larval stage phenotype, non-suppressible by ey^UAS.cHa/Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/bsk^K53R.UAS

(Bunker et al., 2015)

Scer\GAL4^Bx-MS1096, dlg1^HMS01954 has wing disc | third instar larval stage phenotype, non-suppressible by kay^pan-Ala.UAS/Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, dlg1^HMS01954, ey^UAS.cHa has wing disc | third instar larval stage phenotype

(Bunker et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of dlg1^HMS01954 and pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] does not leads to the formation of tumors in the third instar larval wing disc.

(Gerlach et al., 2018)

The tumor phenotype and tissue disorganization resulting from the expression of dlg1^HMS01954 under the control of Scer\GAL4^Bx-MS1096 is fully or partially suppressed by the co-expression of vg^Scer\UAS.cKa, bsk^{K53R.Scer\UAS}, kay^{pan-Ala.Scer\UAS} or brm^{K804R.Scer\UAS.T:Ivir\HA1}, but not by the co-expression of Cat^Scer\UAS.cAa.

The co-expression of ey^Scer\UAS.cHa in third instar larval wing discs already expressing dlg1^HMS01954 under the control of Scer\GAL4^Bx-MS1096 does not suppress the tumor overgrowth, but induces ectopic photoreceptor formation in a small proportion of cells, as compared to controls.

(Bunker et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

39035

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01954}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dlg1^HMS01954

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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