FlyBase Allele Report: Dmel\enok[HMS02165]

General Information

Symbol

Dmel\enok^HMS02165

Species

D. melanogaster

Name

FlyBase ID

FBal0279197

Feature type

allele

Associated gene

Dmel\enok

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS02165}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS02165}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS02165

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{M.UAS.Tag:FLAG}

(Azpurua et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of enok^HMS02165 under the control of the triple Scer\GAL4^nos.PG, Scer\GAL4^{nos.UTR.T:Hsim\VP16}, Scer\GAL4^{otu.T:Hsim\VP16} driver does not cause nurse cell endoreplication defects.

(Huang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/enok^HMS02165, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG is a suppressor | partially of abnormal endomitotic cell cycle | adult stage phenotype of Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG, elg1^HMC03187

(Huang et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/enok^HMS02165, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG is a suppressor | partially of nurse cell phenotype of Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG, elg1^HMC03187

(Huang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The underreplicated nurse cell phenotype characteristic for flies expressing elg1^HMC03187 RNAi under the control of the triple Scer\GAL4^nos.PG, Scer\GAL4^{nos.UTR.T:Hsim\VP16}, Scer\GAL4^{otu.T:Hsim\VP16} driver is partially suppressed by co-expression of enok^HMS02165 RNAi. The nurse cells are still underreplicated but the nuclei are relatively bigger and the cells display higher ploidy levels (they show peaks at at all levels between 64C-1024C) compared to females expressing elg1^HMC03187 alone (show peak only at 64C). Some egg chambers from the double knock-down animals develop beyond stage 9 with morphologically normal nurse cells.

(Huang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

40917