FlyBase Allele Report: Dmel\raptor[HMS02306]

General Information

Symbol

Dmel\raptor^HMS02306

Species

D. melanogaster

Name

FlyBase ID

FBal0279305

Feature type

allele

Associated gene

Dmel\raptor

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS02306}

Also Known As

UAS-raptor-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS02306}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS02306

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by N^int.U.UAS, l(2)gl^GD4047

(Jevitt et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | oogenesis, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

abnormal size | adult stage, with Scer\GAL4^eya.enh1+E+2

(Pletcher et al., 2019)

abnormal stress response | oogenesis, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

decreased cell size | larval stage, with Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Phenotype Manifest In

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

eye, with Scer\GAL4^eya.enh1+E+2

(Pletcher et al., 2019)

female germline stem cell, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

Detailed Description

Statement

Reference

Expression under the control of Scer\GAL4^eya.enh1+E+2 results in a small eye phenotype.

(Pletcher et al., 2019)

Expression of raptor^HMS02306 under the control of Scer\GAL4^Cg.PA does not affect the ability of larvae to pupariate, but does lead to significantly decreased fat body cell size, as compared to controls.

(Schmitt et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased body size | larval stage phenotype, suppressible by Lpin^UAS.cSa, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased body size | larval stage phenotype, suppressible by Lpin^ΔNLS.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has lethal - all die during larval stage phenotype, suppressible by Lpin^UAS.cSa, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has lethal - all die during larval stage phenotype, suppressible by Lpin^ΔNLS.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased cell size | larval stage phenotype, suppressible by Lpin^UAS.cSa, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased cell size | larval stage phenotype, suppressible by Lpin^ΔNLS.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

NOT suppressed by

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased body size | larval stage phenotype, non-suppressible by Lpin^ΔPAP.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has lethal - all die during larval stage phenotype, non-suppressible by Lpin^ΔPAP.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased cell size | larval stage phenotype, non-suppressible by Lpin^ΔPAP.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Other

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased body size | larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has abnormal size | larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased cell size | larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has lethal - all die during larval stage phenotype

(Schmitt et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has embryonic/larval fat body | larval stage phenotype, suppressible by Lpin^UAS.cSa, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has embryonic/larval fat body | larval stage phenotype, suppressible by Lpin^ΔNLS.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

NOT suppressed by

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has embryonic/larval fat body | larval stage phenotype, non-suppressible by Lpin^ΔPAP.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Other

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has nucleus | larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has embryonic/larval fat body | larval stage phenotype

(Schmitt et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Lpin^GD14004 and raptor^HMS02306 under the control of Scer\GAL4^Cg.PA leads to defective larval growth, decreased larval size, extremely small fat body cells with comparatively large nuclei, and failure to pupariate, unlike expression of either Lpin^GD14004 or raptor^HMS02306 alone.

Co-expression of Lpin^Scer\UAS.cSa or Lpin^{ΔNLS.Scer\UAS}, but not Lpin^{ΔPAP.Scer\UAS}, suppresses the larval growth defects, fat body cell size defects, and inability to pupariate seen in larvae expressing Lpin^GD14004 and raptor^HMS02306 under the control of Scer\GAL4^Cg.PA.

(Schmitt et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

41912