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General Information
Symbol
Dmel\ApoltpdsRNA.UAS.II
Species
D. melanogaster
Name
FlyBase ID
FBal0279536
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The FRT cassette has been excised from ApoltpdsRNA.UAS.II, allowing expression of the inverted repeat of Apoltp nucleotides 11525-12082 from the UAS regulatory sequences. The construct targets the ApoltpII protein.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ApoltpdsRNA.Scer\UAS.II in the fat body under the control of Scer\GAL4Adh.PF (initiated by heat shock during the early larval stages) results in dramatic enlargement of the neutral fat droplets in the larval posterior midgut.

Expression of ApoltpdsRNA.Scer\UAS.II under the control of Scer\GAL4Rfabg.PB reduces the larval hemolymph levels of medium-chain diacylglycerol (DAG) (DAG26 and DAG28) and sterols by about 70%. In contrast, levels of PE, the major polar Lpp lipid, are not changed. The amounts of several minor lipid classes (phosphatidylcholine (PC), triacylglycerol (TAG), sphingolipids) increase slightly.

In larval guts expressing ApoltpdsRNA.Scer\UAS.II under the control of Scer\GAL4Adh.PF the levels of medium-chain diacylglycerol (DAG) increase 5-8 fold with respect to polar lipids. Long chain DAG increases moderately, and contributes less to the total elevation in gut DAG. Loss of Apoltp also leads to a strong increase in triacylglycerol (TAG) in the gut, with medium-chain TAG the most strongly elevated. An increase in TAG and DAG is seen in both lipid-fed and lipid-starved animals.

The fat bodies of larvae expressing ApoltpdsRNA.Scer\UAS.II under the control of Scer\GAL4Adh.PF contain much less sterol than those of wild type flies. Fat bodies also contain much less medium-chain diacylglycerol (DAG). Fat body triacylglycerol (TAG) storage is reduced by 30%, but the morphology of neutral lipid droplets is not obviously altered.

Expression of ApoltpdsRNA.Scer\UAS.II under the control of Scer\GAL4Adh.PF specifically depletes levels of medium chain diacylglycerol (DAG) in the wing disc. Long-chain DAG levels are unaffected. Total triacylglycerol (TAG) levels are reduced by 40% and the size and number of neutral lipid droplets are modestly reduced. The reduction in medium-chain TAG levels is more severe than the reduction in long-chain species. Long-chain PE levels are also unaffected.

Expression of ApoltpdsRNA.Scer\UAS.II in the fat body under the control of Scer\GAL4Adh.PF has no effect on neutral lipid levels in the larval brain.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ApoltpdsRNA.Scer\UAS.II
ApoltpdsRNA.UAS.II
Name Synonyms
Secondary FlyBase IDs
    References (1)