FB2025_01 , released February 20, 2025
Allele: Dmel\waplAG.UAS.Tag:HA
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General Information
Symbol
Dmel\waplAG.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0279991
Feature type
allele
Associated gene
Dmel\wapl
Associated Insertion(s)
Carried in Construct
P{UAS-wapl.AG.HA}
Key Links
Transgenic product class
stop_gained
(Cunningham et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
stop_gained
(Cunningham et al., 2012)
Mutagen
in vitro construct
(Cunningham et al., 2012)
Progenitor genotype
waplAG
(Cunningham et al., 2012)
Carried in construct
P{UAS-wapl.AG.HA}
Cytology
Description

UASt regulatory sequences drive expression of a mutated form of wapl containing the same lesion as the waplAG allele (a nonsense mutation at Q271 in the large wapl isoform). The wapl coding sequence is tagged with a Tag:HA epitope.

(Cunningham et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
wapl
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of waplAG.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32 results in a number of mutant phenotypes in adults, including defects in the posterior abdomen, abnormal or extra sternopleural bristles and sex comb teeth on the second legs. Males with a twisted genitalia phenotype are seen.

      (Cunningham et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Scer\GAL4da.G32, waplAG.UAS.Tag:HA has visible phenotype, enhanceable by pds5[+]/pds5e3

      (Cunningham et al., 2012)
      Suppressed by
      Statement
      Reference

      Scer\GAL4da.G32, waplAG.UAS.Tag:HA has visible phenotype, suppressible by Nipped-B29/Nipped-B[+]

      (Cunningham et al., 2012)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Scer\GAL4da.G32, waplAG.UAS.Tag:HA has sex comb | ectopic phenotype, enhanceable by pds5[+]/pds5e3

      (Cunningham et al., 2012)
      Suppressed by
      Statement
      Reference

      Scer\GAL4da.G32, waplAG.UAS.Tag:HA has sex comb | ectopic phenotype, suppressible by Nipped-B29/Nipped-B[+]

      (Cunningham et al., 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      pds5e3/+ enhances the extra sex comb phenotype caused by expression of waplAG.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32.

      Nipped-B29/+ suppresses the extra sex comb phenotype caused by expression of waplAG.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32.

      (Cunningham et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      waplAG.Scer\UAS.T:Ivir\HA1
      waplAG.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)