FakN30/FakK24 larvae show an increase in bouton number per muscle area at the neuromuscular junction compared to wild type.
At 22[o]C, FakN30/FakK24 larvae have enlarged neuromuscular junctions (NMJs) (increased bouton number per muscle area) compared to controls. When reared at 29[o]C, the mutant larvae show no further increase in NMJ size, in contrast to wild-type controls, which show enlarged NMJs at 29[o]C compared to at 22[o]C. The mutant larvae do not show an increase in NMJ size after 8 hours food deprivation, in contrast to controls.
The neuromuscular junction is overgrown compared to wild type in FakN30/FakK24 third instar larvae. The number of boutons per muscle area and NMJ length per muscle area are increased (by 44% and 22% respectively) compared to wild type. There is a reduction in secondary branches and an increase in higher-order branches at the muscle 6/7 NMJ compared to wild type.
The amplitude and frequency of the miniature excitatory junctional potential (mEJP) is normal at the FakN30/FakK24 neuromuscular junction. However, the mean amplitude of the evoked EJP is significantly increased compared to wild type.