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General Information
Symbol
Dmel\FakN30
Species
D. melanogaster
Name
FlyBase ID
FBal0280002
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Fak56N30
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a 3030bp deletion including 2157bp of Fak, 240bp of shared promoter region and 633bp of Spt5.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

FakN30/FakK24 larvae show an increase in bouton number per muscle area at the neuromuscular junction compared to wild type.

At 22[o]C, FakN30/FakK24 larvae have enlarged neuromuscular junctions (NMJs) (increased bouton number per muscle area) compared to controls. When reared at 29[o]C, the mutant larvae show no further increase in NMJ size, in contrast to wild-type controls, which show enlarged NMJs at 29[o]C compared to at 22[o]C. The mutant larvae do not show an increase in NMJ size after 8 hours food deprivation, in contrast to controls.

The neuromuscular junction is overgrown compared to wild type in FakN30/FakK24 third instar larvae. The number of boutons per muscle area and NMJ length per muscle area are increased (by 44% and 22% respectively) compared to wild type. There is a reduction in secondary branches and an increase in higher-order branches at the muscle 6/7 NMJ compared to wild type.

The amplitude and frequency of the miniature excitatory junctional potential (mEJP) is normal at the FakN30/FakK24 neuromuscular junction. However, the mean amplitude of the evoked EJP is significantly increased compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

FakK24/FakN30 has NMJ bouton | supernumerary | larval stage phenotype, non-suppressible by rut1

Other
Additional Comments
Genetic Interactions
Statement
Reference

FakN30/FakKG00304 ; Nf1E2/+ and rut1/+ ; FakN30/FakKG00304 larvae show an increase in bouton number per muscle area at the neuromuscular junction compared to wild type.

Expression of Nf1Scer\UAS.T:Avic\GFP-CFP under the control of Scer\GAL4elav.PU rescues the neuromuscular junction overgrowth phenotype seen in FakN30/FakK24 larvae.

The neuromuscular junction overgrowth phenotype seen in FakN30/FakK24 larvae is unaffected if the larvae are also hemizygous for rut1.

Significant overgrowth of the neuromuscular junction (NMJ) (increased bouton number per muscle area and increased NMJ length per muscle area) is seen in FakN30/FakKG00304 third instar larvae which are also heterozygous for one of βInt-ν1, scb2, Src42AE1 or Src64BPI.

Significant overgrowth of the NMJ (increased bouton number per muscle area) is seen in LanA9-32/+ FakN30/FakKG00304 third instar larvae.

No significant overgrowth of the neuromuscular junction is seen in FakN30/FakKG00304 third instar larvae which are also heterozygous for one of mew1, ifk27e, mys1 or wb4Y18.

The overgrowth at the NMJ (increased bouton number per muscle area) seen in FakN30/FakK24 third instar larvae is enhanced if they are also carrying both Src42AE1/+ and Src64BPI/+.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Comments

The neuromuscular junction defects seen in FakN30/FakK24 larvae are rescued by expression of FakScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU, but not under the control of Scer\GAL4Mhc.PW.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)