Allele Dmel\loqs^{PA.T:Zzzz\FLAG}

General Information
Symbol	Dmel\loqs^{PA.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0280144
Feature type	allele	Associated gene	Dmel\loqs

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Fukunaga et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference loqs promoter and regulatory regions (2.5 kb upstream and 0.8 kb downstream of the locus) drive expression of a cDNA encoding the loqs-PA isoform with a 3xT:Zzzz\FLAG flag added to the amino-terminus of the protein. The construct is in a pattB vector allowing site-specific insertion into the genome. (Fukunaga et al., 2012)
Carried in construct	P{loqs-PA.FLAG} (Fukunaga et al., 2012)
Tagged with	T:Zzzz\FLAG (Fukunaga et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile, with loqs^KO (Fukunaga et al., 2012) female sterile, with loqs^KO, loqs^{PD.T:Zzzz\FLAG} (Fukunaga et al., 2012) male fertile, with loqs^KO (Fukunaga et al., 2012) viable, with loqs^KO (Fukunaga et al., 2012)
Phenotype Manifest In
	dorsal appendage, with loqs^KO (Fukunaga et al., 2012) dorsal appendage, with loqs^KO, loqs^{PD.T:Zzzz\FLAG} (Fukunaga et al., 2012) germline stem cell, with loqs^KO, loqs^{PD.T:Zzzz\FLAG} (Fukunaga et al., 2012)
Detailed Description
	Statement Reference loqs[PA.T:Zzzz\FLAG] restores the embryonic viability of loqs[KO] mutants. The rescued males are fertile, however, females lay far fewer eggs than wild-type controls. None of the eggs produced by loqs[KO] mutants rescued by loqs[PA.T:Zzzz\FLAG] hatch, and few of the eggs display normal dorsal appendages. loqs[KO] mutant females rescued by loqs[PA.T:Zzzz\FLAG] together with loqs[PD.T:Zzzz\FLAG] lay far fewer eggs than wild-type controls. None of the eggs produced by the rescued females hatch. Few of the eggs laid by the rescued females display normal appendages. loqs[KO] mutant females rescued by either loqs[PA.T:Zzzz\FLAG] or the combination of loqs[PA.T:Zzzz\FLAG] and loqs[PD.T:Zzzz\FLAG] display fewer germline stem cells (GSCs) per germaria than wild-type controls. (Fukunaga et al., 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference loqs^{PA.T:Zzzz\FLAG}/loqs^KO is a suppressor \| partially of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs^{PB.T:Zzzz\FLAG}, loqs^{PA.T:Zzzz\FLAG}, loqs^KO is a suppressor \| partially of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012)
NOT Suppressor of
Statement Reference loqs[+], loqs^{PA.T:Zzzz\FLAG}, loqs^KO, loqs^{PD.T:Zzzz\FLAG} is a non-suppressor of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs[+], loqs^{PA.T:Zzzz\FLAG}, loqs^KO is a non-suppressor of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs[+], loqs^{PB.T:Zzzz\FLAG}, loqs^{PA.T:Zzzz\FLAG}, loqs^KO, loqs^{PD.T:Zzzz\FLAG} is a non-suppressor of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs[+], loqs^{PB.T:Zzzz\FLAG}, loqs^{PA.T:Zzzz\FLAG}, loqs^KO is a non-suppressor of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs^{PA.T:Zzzz\FLAG}, loqs^KO, loqs^{PD.T:Zzzz\FLAG} is a non-suppressor of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs^{PB.T:Zzzz\FLAG}, loqs^{PA.T:Zzzz\FLAG}, loqs^KO, loqs^{PD.T:Zzzz\FLAG} is a non-suppressor of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012)
Phenotype Manifest In
Suppressor of
Statement Reference loqs^{PA.T:Zzzz\FLAG}/loqs^KO is a suppressor \| partially of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs^{PB.T:Zzzz\FLAG}, loqs^{PA.T:Zzzz\FLAG}, loqs^KO is a suppressor \| partially of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012)
NOT Suppressor of
Statement Reference loqs[+], loqs^{PA.T:Zzzz\FLAG}, loqs^KO, loqs^{PD.T:Zzzz\FLAG} is a non-suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs[+], loqs^{PA.T:Zzzz\FLAG}, loqs^KO is a non-suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs[+], loqs^{PB.T:Zzzz\FLAG}, loqs^{PA.T:Zzzz\FLAG}, loqs^KO, loqs^{PD.T:Zzzz\FLAG} is a non-suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs[+], loqs^{PB.T:Zzzz\FLAG}, loqs^{PA.T:Zzzz\FLAG}, loqs^KO is a non-suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs^{PA.T:Zzzz\FLAG}, loqs^KO, loqs^{PD.T:Zzzz\FLAG} is a non-suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) loqs^{PB.T:Zzzz\FLAG}, loqs^{PA.T:Zzzz\FLAG}, loqs^KO, loqs^{PD.T:Zzzz\FLAG} is a non-suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	loqs^{PA.T:Zzzz\FLAG}/loqs^{PD.T:Zzzz\FLAG} partially rescues loqs^KO (Fukunaga et al., 2012) loqs^{PA.T:Zzzz\FLAG} partially rescues loqs^KO (Fukunaga et al., 2012)
Comments	loqs[PA.T:Zzzz\FLAG] restores the embryonic viability of loqs[KO] mutants. The rescued males are fertile, however, females lay far fewer eggs than wild-type controls. None of the eggs produced by loqs[KO] mutants rescued by loqs[PA.T:Zzzz\FLAG] hatch, and few of the eggs display normal dorsal appendages. loqs[KO] mutant females rescued by loqs[PA.T:Zzzz\FLAG] together with loqs[PD.T:Zzzz\FLAG] lay far fewer eggs than wild-type controls. None of the eggs produced by the rescued females hatch. Few of the eggs laid by the rescued females display normal appendages. loqs[KO] mutant females rescued by either loqs[PA.T:Zzzz\FLAG] or the combination of loqs[PA.T:Zzzz\FLAG] and loqs[PD.T:Zzzz\FLAG] display fewer germline stem cells (GSCs) per germaria than wild-type controls. (Fukunaga et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	loqs^{PA.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Fukunaga et al., 2012, Cell 151(3): 533--546 Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals. [FBrf0219804]

Allele Dmel\loqsPA.T:Zzzz\FLAG

Allele Dmel\loqs^{PA.T:Zzzz\FLAG}