Allele Dmel\tn^ΔA

General Information
Symbol	Dmel\tn^ΔA	Species	D. melanogaster
Name		FlyBase ID	FBal0280149
Feature type	allele	Associated gene	Dmel\tn

Allele class	amorphic allele - molecular evidence
Mutagen	minos transposase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - molecular evidence (Labeau-Dimenna et al., 2012)
Mutagen	minos transposase (Labeau-Dimenna et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Mi{ET1}tn^MB03490 (Labeau-Dimenna et al., 2012)
Nature of the lesion	Statement Reference Excision of the Mi{ET1}abba[MB03490] element resulting in the tn[ΔA] deletion allele. (Labeau-Dimenna et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with Df(2R)Exel6068) (Labeau-Dimenna et al., 2012) lethal (with tn¹) (Labeau-Dimenna et al., 2012) visible \| pupal stage (with Df(2R)Exel6068) (Labeau-Dimenna et al., 2012) visible \| pupal stage (with tn¹) (Labeau-Dimenna et al., 2012)
Phenotype Manifest In
	embryonic/larval somatic muscle \| third instar larval stage (with Df(2R)Exel6068) (Labeau-Dimenna et al., 2012) myofibril \| third instar larval stage (with Df(2R)Exel6068) (Labeau-Dimenna et al., 2012)
Detailed Description
	Statement Reference tn[ΔA]/Df(2R)Exel6068 pupae are thin and long. Compared to wild type, mutant L3 larval skeletal musculature is thinner and looser. At higher magnification, mutant muscles show myofiber instability and unbundling. tn[1]/tn[ΔA] pupae are thin and long. A large proportion of tn[1]/tn[ΔA] and Df(2R)Exel6068/tn[ΔA] animals die during embryogenesis; either no or very low numbers are lost during larval stages; animals that survive the larval stages die as pupae. Myofibrils of tn[ΔA]/Df(2R)Exel6068 larval muscle cells become unbundled directionally: the muscle unbundling is more severe towards the visceral surface of the muscle and retains a more wild type appearance toward the most lateral region of the cell. Also, muscle in older individuals appears to have a greater degree of degeneration than that of younger individuals, suggesting progressive muscle degeneration. (Labeau-Dimenna et al., 2012)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhanced by
Statement Reference tn^ΔA/tn¹ has visible \| pupal stage phenotype, non-enhanceable by Df(3R)Mlp84B^P8/+ (Labeau-Dimenna et al., 2012) tn^ΔA/tn¹ has visible \| pupal stage phenotype, non-enhanceable by sls[+]/sls^j1D7 (Labeau-Dimenna et al., 2012)
NOT Enhancer of
Statement Reference tn[+]/tn^ΔA is a non-enhancer of visible \| pupal stage phenotype of sls^g65/sls^j1D7 (Labeau-Dimenna et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Df(3R)Mlp84B[P8]/+ or sls[j1D7]/+ does not enhance the tn[1]/tn[ΔA] thin pupae phenotype. tn[ΔA]/+ does not enhance the sls[j1D7]/sls[g65] thin pupae phenotype. (Labeau-Dimenna et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Df(2R)Exel6068/tn^ΔA is rescued by Scer\GAL4^how-24B/tn^Scer\UAS.cLa (Labeau-Dimenna et al., 2012)
Comments	Scer\GAL4[how-24B]-mediated expression of tn[Scer\UAS.cLa] rescues the thin pupal phenotype, the thin and loose larval skeletal musculature, and larval myofiber instability and unbundling phenotypes of tn[ΔA]/Df(2R)Exel6068 animals. Unlike tn[ΔA]/Df(2R)Exel6068 animals, the rescued animals mostly eclose as adults. (Labeau-Dimenna et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	tn^ΔA (Labeau-Dimenna et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Labeau-Dimenna et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(44): 17983--17988 Thin, a Trim32 ortholog, is essential for myofibril stability and is required for the integrity of the costamere in Drosophila. [FBrf0219862]

Allele Dmel\tnΔA

Allele Dmel\tn^ΔA