FlyBase Allele Report: Dmel\RpL8[RNAi.UAS]

General Information

Symbol

Dmel\RpL8^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0280384

Feature type

allele

Associated gene

Dmel\RpL8

Associated Insertion(s)

Carried in Construct

P{UAS-RpL8.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Xu et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Xu et al., 2008)

Mutagen

in vitro construct

(Xu et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-RpL8.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a RpL8 RNAi line.

(Xu et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

RpL8

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size | adult stage | somatic clone, with Scer\GAL4^FRT.Act5C

(Xu et al., 2008)

visible, with Scer\GAL4^GMR.PF

(Xu et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Xu et al., 2008)

ommatidium, with Scer\GAL4^GMR.PF

(Xu et al., 2008)

wing margin bristle | somatic clone, with Scer\GAL4^FRT.Act5C

(Xu et al., 2008)

Detailed Description

Statement

Reference

When RpL8^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF the eyes are small compared to wild type and appear flattened. This phenotype is not due to a reduction in the number of ommatidia, but due to a reduction in their size.

The wing margin bristle cells in clones expressing RpL8^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Scer\FRT.Act5C} are reduced in size by 20% compared to controls.

(Xu et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Hsap\RPL8^UAS.cXa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PF/RpL8^RNAi.UAS is an enhancer of visible | adult stage | somatic clone phenotype of S6k^l-1

(Xu et al., 2008)

Scer\GAL4^GMR.PF/RpL8^RNAi.UAS is an enhancer of abnormal size | adult stage | somatic clone phenotype of S6k^l-1

(Xu et al., 2008)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/RpL8^RNAi.UAS is a suppressor of visible | somatic clone | adult stage phenotype of Tsc1^Q600X

(Xu et al., 2008)

Scer\GAL4^GMR.PF/RpL8^RNAi.UAS is a suppressor of abnormal size | somatic clone | adult stage phenotype of Tsc1^Q600X

(Xu et al., 2008)

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor of abnormal size | adult stage phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of visible | adult stage phenotype of S6k^UAS.cWa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of abnormal size | adult stage phenotype of S6k^UAS.cWa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hsap\RPL8^UAS.cXa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Hsap\RPL8^UAS.cXa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PF/RpL8^RNAi.UAS is an enhancer of eye | somatic clone phenotype of S6k^l-1

(Xu et al., 2008)

Suppressor of

Statement

Reference

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of ommatidium phenotype of InR^UAS.cHa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of ommatidium phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Xu et al., 2008)

RpL8^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of S6k^UAS.cWa, Scer\GAL4^GMR.PF

(Xu et al., 2008)

Scer\GAL4^GMR.PF/RpL8^RNAi.UAS is a suppressor of eye | somatic clone phenotype of Tsc1^Q600X

(Xu et al., 2008)

Scer\GAL4^GMR.PF/RpL8^RNAi.UAS is a suppressor of adult head capsule | somatic clone phenotype of Tsc1^Q600X

(Xu et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of RpL8^{dsRNA.Scer\UAS} partially suppresses the increase in ommatidia size seen when InR^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of RpL8^{dsRNA.Scer\UAS} partially suppresses the increase in ommatidia size seen when Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of RpL8^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF partially suppresses the increase in eye and head capsule size seen in Tsc1^Q600X mutant clones.

Expression of RpL8^{dsRNA.Scer\UAS} partially suppresses the increase in eye size seen when S6k^Scer\UAS.cWa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of RpL8^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF enhances the reduction in eye size seen in S6k^l-1 mutant clones.

Expression of RpL8^{dsRNA.Scer\UAS} partially suppresses the increase in eye size seen when dm^Scer\UAS.cZa is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\RPL8^Scer\UAS.cXa completely suppresses the small eye phenotype seen when RpL8^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

Complementation and Rescue Data

Rescued by

RpL8^RNAi.UAS is rescued by Scer\GAL4^GMR.PF/RpL8^RNAi.UAS

(Xu et al., 2008)

Rescues

Scer\GAL4^GMR.PF/RpL8^RNAi.UAS rescues RpL8^RNAi.UAS

(Xu et al., 2008)

Comments

Expression of RpL8^Scer\UAS.cXa completely rescues the small eye phenotype seen when RpL8^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RpL8^RNAi.UAS

RpL8^{dsRNA.Scer\UAS}

RpL8^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

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