The reduced total neuromuscular junction (NMJ) bouton area in third instar larvae expressing Hsap\DISC1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4αTub84B.PL is rescued by combination with a single copy of Nrx-1d08766 or co-expression of Nrx-1JF02652 but this also results in significant decrease in the number of branch points per NMJ, which is not observed in any of the single mutants alone. Number of boutons per NMJ remains comparable to wild-type.
Similarly, Scer\GAL4αTub84B.PL>Hsap\DISC1Scer\UAS.T:Ivir\HA1;Nrx-1d08766/+ larvae show significant decrease in both average and peak locomotor speed, a phenotype not seen in either of the single mutants. Cell body size of neurons in the ventral nerve cord is not affected and comparable to wild-type.
The neuromuscular junction bouton phenotype characteristic for third instar larvae expressing Hsap\DISC1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4tub.PU is enhanced by combination with a single copy of the Dysbe01028 allele: not only is the total bouton areas decreased further, in addition the total number of boutons as well as their individual size is significantly reduced compared to wild-type, whereas the number of axonal branching points remains normal.