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General Information
Symbol
Dmel\orb2::Mmus\Cpeb2RBD.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0280517
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
A modified genomic fragment has been inserted into the attP site present in orb2attP via phiC31-mediated recombination. The resulting allele consists of orb2 tagged at the C-terminal end with GFP, in which the orb2 RBD domain has been replaced by the Mmus\Cpeb2 RBD domain. A single mFRT11 site is present downstream of the orb2 coding sequence, and single attL and attR sites (generated during the recombination process) are also present.
Insertion components
TI{TI}orb2::Mmus\Cpeb2RBD.GFP
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Homozygous orb2::Mmus\Cpeb2RBD.T:Avic\GFP male flies show strong defects in long term 24 hour memory when exposed to a courtship conditioning paradigm. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females often fail to suppress their courtship towards other mated females. Short term 1 hour memory is comparable to wild type.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Expression of orb2Scer\UAS.B.cKa under the control of Scer\GAL4Mef2.247 (restricted to the adult stages using Scer\GAL80ts.αTub84B) rescues the long term memory defects seen in orb2::Mmus\Cpeb2RBD.T:Avic\GFP mutant flies exposed to a courtship conditioning paradigm. Expression of orb2RBD.Scer\UAS.B under the control of Scer\GAL4Mef2.247 (restricted to the adult stages using Scer\GAL80ts.αTub84B) fails to rescue the long term memory defects seen in orb2::Mmus\Cpeb2RBD.T:Avic\GFP mutant flies exposed to a courtship conditioning paradigm. orb2::Mmus\Cpeb2RBD.T:Avic\GFP/orb2ΔA.T:Avic\GFP male flies have no long term 24 hour memory when exposed to a courtship conditioning paradigm. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females fail to suppress their courtship towards other mated females. Short term 1 hour memory is comparable to wild type. orb2::Mmus\Cpeb2RBD.T:Avic\GFP/orb2ΔB.T:Avic\GFP male flies have almost no long term 24 hour memory when exposed to a courtship conditioning paradigm. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females fail to suppress their courtship towards other mated females. Short term 1 hour memory is comparable to wild type. orb2RBDΔA.T:Avic\GFP/orb2::Mmus\Cpeb2RBD.T:Avic\GFP male flies have almost no long term 24 hour memory when exposed to a courtship conditioning paradigm. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females fail to suppress their courtship towards other mated females.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
orb2::Mmus\Cpeb2RBD.GFP
orb2::Mmus\Cpeb2RBD.T:Avic\GFP
orb2mCPEB2RBD
Name Synonyms
Secondary FlyBase IDs
    References (3)