FlyBase Allele Report: Dmel\Sema1a[UAS.cJa]

General Information

Symbol

Dmel\Sema1a^UAS.cJa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Jeong

FlyBase ID

FBal0280755

Feature type

allele

Associated gene

Dmel\Sema1a

Associated Insertion(s)

Carried in Construct

PBac{UAS-Sema1a.J}

Also Known As

UAS-Sema-1a

Key Links

Transgenic product class

wild_type

(Jeong et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Jeong et al., 2012)

Mutagen

in vitro construct

(Jeong et al., 2012)

Progenitor genotype

Carried in construct

PBac{UAS-Sema1a.J}

Cytology

Description

UAS regulatory sequences drive expression of wild-type Sema1a.

(Jeong et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Sema1a

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Sema1a^Scer\UAS.cJa under the control of Scer\GAL4^elav.PLu in embryos does not induce significant increase in axon pathfinding defects relative to controls.

(Jeong et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, Sema1a^UAS.cJa, pbl^UAS.N.Tag:HA has abnormal neuroanatomy | embryonic stage phenotype, suppressible by PlexA^MB09499/plexA[+]

(Jeong et al., 2012)

Enhancer of

Statement

Reference

Sema1a^UAS.cJa/Scer\GAL4^elav.PLu is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^elav.PLu, pbl^UAS.N.Tag:HA

(Jeong et al., 2017)

Other

Statement

Reference

Scer\GAL4^elav.PLu, Sema1a^UAS.cJa, pbl^UAS.N.Tag:HA has abnormal neuroanatomy | embryonic stage phenotype

(Jeong et al., 2017, Jeong et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, Sema1a^UAS.cJa, pbl^UAS.N.Tag:HA has lateral longitudinal fascicle phenotype, suppressible by PlexA^MB09499/plexA[+]

(Jeong et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, Sema1a^UAS.cJa, pbl^UAS.N.Tag:HA has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-suppressible by PlexA^MB09499/plexA[+]

(Jeong et al., 2012)

Enhancer of

Statement

Reference

Sema1a^UAS.cJa/Scer\GAL4^elav.PLu is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Scer\GAL4^elav.PLu, pbl^UAS.N.Tag:HA

(Jeong et al., 2017)

Sema1a^UAS.cJa/Scer\GAL4^elav.PLu is an enhancer of axon | embryonic stage phenotype of Scer\GAL4^elav.PLu, pbl^UAS.N.Tag:HA

(Jeong et al., 2017)

Other

Statement

Reference

Scer\GAL4^elav.PLu, Sema1a^UAS.cJa, pbl^UAS.N.Tag:HA has axon | embryonic stage phenotype

(Jeong et al., 2017)

Scer\GAL4^elav.PLu, Sema1a^UAS.cJa, pbl^UAS.N.Tag:HA has presumptive embryonic/larval central nervous system | embryonic stage phenotype

(Jeong et al., 2017)

Scer\GAL4^elav.PLu, Sema1a^UAS.cJa, pbl^UAS.N.Tag:HA has lateral longitudinal fascicle phenotype

(Jeong et al., 2012)

Scer\GAL4^elav.PLu, Sema1a^UAS.cJa, pbl^UAS.N.Tag:HA has larval intersegmental nerve branch ISNb of A1-7 phenotype

(Jeong et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The moderate axon pathfinding defects (defasciculation defects in intersegmental nerve b motor axons very infrequent lateral axon tract disruptions in the central nervous system) characteristic for embryos expressing pbl^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^elav.PLu are strongly exacerbated by co-expression of Sema1a^Scer\UAS.cJa and in addition, the embryos also display midline crossing defects.

(Jeong et al., 2017)

Co-expression of Sema-1a^Scer\UAS.cJa and pbl^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^elav.PLu results in ISNb defects in 46.1% of hemisegments. Defects in the lateral-most Fas2-positive longitudinal connectives are seen in 21.6% of hemisegments. An average of 3.6 ectopic midline crossings in the central nervous system are seen per embryo.

plexA^MB09499/+ strongly suppresses the lateral longitudinal central nervous system defects seen in embryos co-expressing Sema-1a^Scer\UAS.cJa and pbl^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^elav.PLu, while the ISNb defects are not suppressed.

(Jeong et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Sema1a^UAS.cJa/Scer\GAL4^sca-537.4 partially rescues Sema1a^k13702

(Jeong et al., 2017)

Sema1a^UAS.cJa/Scer\GAL4^sca-537.4 partially rescues Sema1a^k13702

(Jeong et al., 2012)

Comments

Expression of Sema1a^Scer\UAS.cJa under the control of Scer\GAL4^sca-537.4 strongly rescues the axon guidance defects characteristic for Sema1a^k13702 homozygous embryos - the defects in the central nervous system are completely suppressed but the frequency of defasciculation irregularities in the intersegmental nerve b is only partially decreased and remains elevated compared to wild-type controls.

(Jeong et al., 2017)

Expression of Sema-1a^Scer\UAS.cJa under the control of Scer\GAL4^sca-537.4 rescues the central nervous system guidance defects and partially rescues the ISNb guidance defects seen in Sema-1a^k13702 embryos.

(Jeong et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

65734

y¹ w^*; PBac{UAS-Sema1a.J}VK00033

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sema-1a^Scer\UAS.cJa

Sema1a^Scer\UAS.cJa

Sema1a^UAS.cJa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Jeong

Secondary FlyBase IDs

References (4)

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